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- BOSTER
- A03283-3-100ul
- 中国
- 2025年07月11日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A03283-3 |
|---|---|
| 产品名称 | Anti-GNL3 Antibody |
| 基因名 | GNL3 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 65KD |
| 免疫原 | E.coli-derived human Nucleostemin/GNL3 recombinant protein (Position: R45-V549). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Guanine nucleotide-binding protein-like 3, also known as nucleostemin, is a protein that in humans is encoded by the GNL3 gene. The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. |
| 研究类别 | 1. Charpentier, A. H., Bednarek, A. K., Daniel, R. L., Hawkins, K. A., Laflin, K. J., Gaddis, S., MacLeod, M. C., Aldaz, C. M. Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Cancer Res. 60: 5977-5983, 2000. 2. Dai, M.-S., Sun, X.-X., Lu, H. Aberrant expression of nucleostemin activates p53 and induces cell cycle arrest via inhibition of MDM2. Molec. Cell. Biol. 28: 4365-4376, 2008. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 6/29/2012. |
| Uniprot ID | GNL3: Q9BVP2 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- Nucleostemin/GNL3 Antibod (A03283-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Jurkat whole cell lysates,
Lane 5: C6 whole cell lysates,
Lane 6: NIH/3T3 whole cell lysates.
Use rabbit anti- GNL3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GNL3 at approximately 65KD. The expected band size for GNL3 is at 62KD.|Figure 2. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human appendiceal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. ICC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). was detected in immersion fixed HEP3B cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of HeLa cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Jurkat whole cell lysates,
Lane 5: C6 whole cell lysates,
Lane 6: NIH/3T3 whole cell lysates.
Use rabbit anti- GNL3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GNL3 at approximately 65KD. The expected band size for GNL3 is at 62KD.|Figure 2. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human appendiceal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). detected in paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. ICC analysis using anti- Nucleostemin/GNL3 Antibod (A03283-3). was detected in immersion fixed HEP3B cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of HeLa cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Nucleostemin/GNL3 Antibody(A03283-3-100ul)
￥1960
