- ￥1960
- BOSTER
- A04228-2-100ul
- 中国
- 2025年07月15日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A04228-2 |
|---|---|
| 产品名称 | Anti-RAE1 Antibody |
| 基因名 | RAE1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 41KD |
| 免疫原 | E.coli-derived human RAE1 recombinant protein (Position: Q76-K368). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | mRNA export factor is a protein that in humans is encoded by the RAE1 gene. Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| 研究类别 | 1. Bharathi, A., Ghosh, A., Whalen, W. A., Yoon, J. H., Pu, R., Dasso, M., Dhar, R. The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of poly(A)+ RNA. Gene 198: 251-258, 1997. 2. Jeganathan, K. B., Malureanu, L., van Deursen, J. M. The Rae1-Nup98 complex prevents aneuploidy by inhibiting securin degradation. Nature 438: 1036-1039, 2005. 3. Kraemer, D., Blobel, G. mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm. Proc. Nat. Acad. Sci. 94: 9119-9124, 1997. |
| Uniprot ID | RAE1: P78406 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- RAE1 Antibody (A04228-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat thymus tissue lysates,
Use rabbit anti- RAE1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RAE1 at approximately 41KD. The expected band size for RAE1 is at 41KD.|Figure 2. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. ICC analysis using anti- RAE1 Antibody (A04228-2). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat thymus tissue lysates,
Use rabbit anti- RAE1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RAE1 at approximately 41KD. The expected band size for RAE1 is at 41KD.|Figure 2. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human Gall bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- RAE1 Antibody (A04228-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. ICC analysis using anti- RAE1 Antibody (A04228-2). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-RAE1 Antibody(A04228-2-100ul)
￥1960
