- ￥1960
- BOSTER
- A15075-1-100ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A15075-1 |
|---|---|
| 产品名称 | Anti-ZC3H7A Antibody |
| 基因名 | ZC3H7A |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 111KD |
| 免疫原 | E.coli-derived human ZC3H7A recombinant protein (Position: Q13-R717). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ZC3H7A (Zinc Finger CCCH-Type Containing 7A, also known as ZC3H7; HSPC055; ZC3HDC7), a member of CCCH gene family, is located on human chromosome 16p13.13. ZC3H7A is associated with some diseases, including Endometrial Stromal Tumor and Endometrial Stromal Sarcoma. An important paralog of this gene is ZC3H7B. The CCCH zinc finger motif has been found in proteins from organisms ranging from man to yeast. The CCCH proteins are a large family of zinc finger containing C3H-type motifs and much evidences proved that they may be RNA-binding proteins functioning in RNA processing. In mouse, tristetraprolin, a protein containing two CCCH zinc fingers, binds directly to AU-rich elements within the 3'-untranslated region of target transcripts to facilitate mRNA degradation. Zfp36l2, like its better-known relative TTP, is a mRNA-binding and destabilizing protein, functions in the physiological control of female fertility at the level of early embryonic development. The PIE-1 is an essential regulator of Caenorhabditis elegans germ cell fate that segregates with the germ lineage by inhibition of transcription or activation of protein expression from maternal RNAs. |
| 研究类别 | 1. Wang, D. , Guo, Y. , Wu, C. , Yang, G. , Li, Y. , & Zheng, C. . (2008). Genome-wide analysis of ccch zinc finger family in arabidopsis and rice. Bmc Genomics, 9(1), 44.2. Jian, L. , Song, W. , Gail, T. , Kolattukudy, P. E. , Fu, M. , & Mark, I. . (2008). Genome-wide survey and expression profiling of ccch-zinc finger family reveals a functional module in macrophage activation. PLoS ONE, 3(8), e2880. |
| Uniprot ID | ZC3H7A: Q8IWR0 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- ZC3H7A Antibody (A15075-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: K562 whole cell lysates.
Use rabbit anti- ZC3H7A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ZC3H7A at approximately 111KD. The expected band size for ZC3H7A is at 111KD.|Figure 2. ICC analysis using anti- ZC3H7A Antibody (A15075-1). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 3. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: K562 whole cell lysates.
Use rabbit anti- ZC3H7A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ZC3H7A at approximately 111KD. The expected band size for ZC3H7A is at 111KD.|Figure 2. ICC analysis using anti- ZC3H7A Antibody (A15075-1). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 3. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-ZC3H7A Antibody(A15075-1-100ul)
￥1960
