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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | A05296-1 |
|---|---|
| 产品名称 | Anti-ALDOC Antibody |
| 基因名 | ALDOC |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 39KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human Aldolase C/ALDOC (QKDDNGVPFVRTIQDK). |
| 内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Aldolase C, fructose-bisphosphate (ALDOC, or ALDC), is an enzyme that, in humans, is encoded by the ALDOC gene on chromosome 17. This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. |
| 研究类别 | 1. Buono, P., Paolella, G., Mancini, F. P., Izzo, P., Salvatore, F. The complete nucleotide sequence of the gene coding for the human aldolase C. Nucleic Acids Res. 16: 4733 only, 1988. 2. Rocchi, M., Vitale, E., Covone, A., Romeo, G., Santamaria, R., Buono, P., Paolella, G., Salvatore, F. Assignment of human aldolase C gene to chromosome 17, region cen--q21.1. Hum. Genet. 82: 279-282, 1989.3. Rottmann, W. H., Deselms, K. R., Niclas, J., Camerato, T., Holman, P. S., Green, C. J., Tolan, D. R. The complete amino acid sequence of the human aldolase C isozyme derived from genomic clones. Biochimie 69: 137-145, 1987. |
| Uniprot ID | ALDOC: P09972 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- Aldolase C/ALDOC Antibody (A05296-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use rabbit anti- ALDOC 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ALDOC at approximately 39KD. The expected band size for ALDOC is at 39KD.|Figure 2. Flow cytometry analysis of C2C12 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 3. Flow cytometry analysis of RH35 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use rabbit anti- ALDOC 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ALDOC at approximately 39KD. The expected band size for ALDOC is at 39KD.|Figure 2. Flow cytometry analysis of C2C12 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 3. Flow cytometry analysis of RH35 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Aldolase C/ALDOC Antibody(A05296-1-100ul)
¥1960







