- ￥1960
- BOSTER
- A14815-100ul
- 中国
- 2025年07月11日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A14815 |
|---|---|
| 产品名称 | Anti-ARL6IP6 Antibody |
| 基因名 | ARL6IP6 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 22KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human ARL6IP6 (VARDLRAEFSAGA). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ADP ribosylation factor like GTPase 6 interacting protein 6 is a protein that in the humans is encoded by the ARL6IP6 gene. ARL6IP6 (ADP-ribosylation-like factor 6 interacting protein 6), also known as PFAAP1 (phosphonoformate immuno-associated protein 1), is a 226 amino acid multi-pass membrane protein that belongs to the ARL6IP6 family and is encoded by a gene that maps to human chromosome 2q23.3. A 2q23.3 novel microdeletion involving seven genes, including ARL6IP6, is linked to a proposed 2q23q24 microdeletion syndrome. Additional chromosome 2q deletions, which also include ARL6IP6, are linked to autism, developmental delays and communication impairment. As the second largest human chromosome, chromosome 2 makes up approximately 8% of the human genome and contains 237 million bases encoding over 1,400 genes. Chromosome 2 contains a probable vestigial second centromere, as well as vestigial telomeres, which gives credence to the hypothesis that human chromosome 2 formed as a result of an ancient fusion of two ancestral chromosomes, which are stillpresent in modern day apes. |
| 研究类别 | 1. Abumansour, I. S., Hijazi, H., Alazmi, A., Alzahrani, F., Bashiri, F. A., Hassan, H., Alhaddab, M., Alkuraya, F. S. ARL6IP6, a susceptibility locus for ischemic stroke, is mutated in a patient with syndromic cutis marmorata telangiectatica congenita. Hum. Genet. 134: 815-822, 2015. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/28/2015.3. Ingley, E., Williams, J. H., Walker, C. E., Tsai, S., Colley, S., Sayer, M. S., Tilbrook, P. A., Sarna, M., Beaumont, J. G., Klinken, S. P. A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61-beta subunit. FEBS Lett. 459: 69-74, 1999. |
| Uniprot ID | ARL6IP6: Q8N6S5 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti- ARL6IP6 Antibody (A14815). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates.
Lane 2: Caco-2 whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse testis tissue lysates,
Lane 5: mouse lung tissue lysates.
Use rabbit anti- ARL6IP6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ARL6IP6 at approximately 22KD. The expected band size for ARL6IP6 is at 25KD.|Figure 2. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human girac cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 7. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 8. ICC analysis using anti- ARL6IP6 Antibody (A14815). was detected in immersion fixed CACO-2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).|Figure 9. Flow cytometry analysis of HEPA1-6 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 10. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: 293T whole cell lysates.
Lane 2: Caco-2 whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse testis tissue lysates,
Lane 5: mouse lung tissue lysates.
Use rabbit anti- ARL6IP6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ARL6IP6 at approximately 22KD. The expected band size for ARL6IP6 is at 25KD.|Figure 2. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human girac cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 7. IHC analysis using anti- ARL6IP6 Antibody (A14815). detected in paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 8. ICC analysis using anti- ARL6IP6 Antibody (A14815). was detected in immersion fixed CACO-2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).|Figure 9. Flow cytometry analysis of HEPA1-6 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 10. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Anti-ARL6IP6 Antibody(A14815-100ul)
￥1960
