- ￥1960
- BOSTER
- M02602-2-100ul
- 中国
- 2025年07月09日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M02602-2 |
|---|---|
| 产品名称 | Anti-SND1 Antibody (monoclonal, 6G3B4) |
| 基因名 | SND1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:6G3B4) |
| 抗体亚型 | Mouse IgG2a |
| 分子量 | 110KD |
| 免疫原 | E.coli-derived human SND1 recombinant protein (Position: Q20-D204). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Staphylococcal nuclease domain-containing protein 1 also known as 100 kDa coactivator or Tudor domain-containing protein 11 (TDRD11) is a protein that in humans is encoded by the SND1 gene. This gene encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC). |
| 研究类别 | 1. Callebaut, I., Mornon, J. P. The human EBNA-2 coactivator p100: multidomain organization and relationship to the staphylococcal nuclease fold and to the tudor protein involved in Drosophila melanogaster development. Biochem. J. 321: 125-132, 1997. 2. Caudy, A. A., Ketting, R. F., Hammond, S. M., Denli, A. M., Bathoorn, A. M. P., Tops, B. B. J., Silva, J. M., Myers, M. M., Hannon, G. J., Plasterk, R. H. A. A micrococcal nuclease homologue in RNAi effector complexes. Nature 425: 411-414, 2003. 3. Elbarbary, R. A., Miyoshi, K., Myers, J. R., Du, P., Ashton, J. M., Tian, B., Maquat, L. E. Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G1/S phase transition. Science 356: 859-862, 2017. |
| Uniprot ID | SND1: Q7KZF4 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SND1 Antibody (M02602-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat pancrease tissue lysates,
Lane 6: mouse stomach tissue lysates,
Lane 7: mouse pancrease tissue lysates.
Use mouse anti- SND1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SND1 at approximately 110KD. The expected band size for SND1 is at 102KD.|Figure 2. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of human Metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of rectum adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. ICC analysis using anti- SND1 Antibody (M02602-2). was detected in immersion fixed PC-3 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 10. Flow cytometry analysis of HeLa cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat pancrease tissue lysates,
Lane 6: mouse stomach tissue lysates,
Lane 7: mouse pancrease tissue lysates.
Use mouse anti- SND1 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SND1 at approximately 110KD. The expected band size for SND1 is at 102KD.|Figure 2. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of human Metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of rectum adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SND1 Antibody (M02602-2). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. ICC analysis using anti- SND1 Antibody (M02602-2). was detected in immersion fixed PC-3 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 10. Flow cytometry analysis of HeLa cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SND1 Antibody (monoclonal, 6G3B4)(M02602-2-100ul)
￥1960
