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- BOSTER
- A06891-1-100ul
- 中国
- 2025年07月15日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A06891-1 |
|---|---|
| 产品名称 | Anti-Psmb1 Antibody |
| 基因名 | Psmb1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 26KD |
| 免疫原 | E.coli-derived mouse Psmb1 recombinant protein (Position: S21-D240). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Proteasome subunit beta type-1 also known as 20S proteasome subunit beta-6 (based on systematic nomenclature) is a protein that in humans is encoded by the PSMB1 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. |
| 研究类别 | 1. Okumura, K., Nogami, M., Taguchi, H., Hisamatsu, H., Tanaka, K. The genes for the alpha-type HC3 (PMSA2) and beta-type HC5 (PMSB1) subunits of human proteasomes map to chromosomes 6q27 and 7p12-p13 by fluorescence in situ hybridization. Genomics 27: 377-379, 1995. 2. Tamura, T., Osaka, F., Kawamura, Y., Higuti, T., Ishida, N., Nothwang, H.-G., Tsurumi, C., Tanaka, K., Ichihara, A. Isolation and characterization of alpha-type HC3 and beta-type HC5 subunit genes of human proteasomes. J. Molec. Biol. 244: 117-124, 1994. 3. Trachtulec, Z., Hamvas, R. M. J., Forejt, J., Lehrach, H. R., Vincek, V., Klein, J. Linkage of TATA-binding protein and proteasome subunit C5 genes in mice and humans reveals synteny conserved between mammals and invertebrates. Genomics 44: 1-7, 1997. |
| Uniprot ID | Psmb1: O09061 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-Psmb1 Antibody (A06891-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: C6 whole cell lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: NIH3T3 whole cell lysates,
Use rabbit anti-smb1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Psmb1 at approximately 26KD. The expected band size for Psmb1 is at 26KD.|Figure 2.low cytometry analysis of RAW264.7 (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: rat stomach tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: C6 whole cell lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: NIH3T3 whole cell lysates,
Use rabbit anti-smb1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Psmb1 at approximately 26KD. The expected band size for Psmb1 is at 26KD.|Figure 2.low cytometry analysis of RAW264.7 (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Psmb1 Antibody(A06891-1-100ul)
￥1960
