E.coli-derived human SHISA6 recombinant protein (Position: K253-Y483).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
The deduced 551-amino acid human SHISA6 protein has a domain structure typical of SHISA proteins, including an N-terminal signal peptide, followed by a cysteine-rich domain, a transmembrane domain, and a C-terminal proline-rich region. SHISA6 belongs to a SHISA subfamily containing SHISA7, SHISA8, and SHISA9. The SHISA6 gene is mapped to chromosome 17p12 based on an alignment of the SHISA6 sequence (GenBank AK127379) with the genomic sequence (GRCh38).
研究类别
1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/30/2017. 2. Pei, J., Grishin, N. V. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell. Signal. 24: 758-769, 2012.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of anti- SHISA6 antibody (A14975-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.Use rabbit anti- SHISA6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SHISA6 at approximately 56KD. The expected band size for SHISA6 at 56KD.|Figure 2. Flow cytometry analysis of U2OS cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]