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100ul
货号 | A05331 |
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产品名称 | Anti-GLRX2 Antibody |
基因名 | GLRX2 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 18KD |
免疫原 | E.coli-derived human GRX2/GLRX2 recombinant protein (Position: M1-Q164). |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | GLRX2(Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins(e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al.(2001) and Gladyshev et al.(2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al.(2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon(exon 1B) located upstream from the first exon used by the GLRX2A transcript(exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al.(2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity. |
研究类别 | 1. Gladyshev, V. N., Liu, A., Novoselov, S. V., Krysan, K., Sun, Q.-A., Kryukov, V. M., Kryukov, G. V., Lou, M. F.Identification and characterization of a new mammalian glutaredoxin (thioltransferase), Grx2.J. Biol. Chem. 276: 30374-30380, 2001.2. Lilig, C. H., Berndt, C., Vergnolle, O., Lonn, M. E., Hudemann, C., Bill, E., Holmgren, A.Characterization of human glutaredoxin 2 as iron-sulfur protein: a possible role as redox sensor.Proc. Nat. Acad. Sci. 102: 8168-8173, 2005.3. Lundberg, M., Johansson, C., Chandra, J., Enoksson, M., Jacobsson, G., Ljung, J., Johansson, M., Holmgren, A.Cloning and expression of a novel human glutaredoxin (Grx2) with mitochondrial and nuclear isoforms.J. Biol. Chem. 276: 26269-26275, 2001. |
Uniprot ID | GLRX2: Q9NS18 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot(WB): | 1:500-2000 |
Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Flow cytometry (FCM): | 1-3 μg/1x106 cells |
(ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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