- ￥1960
- BOSTER
- A02975-2-100ul
- 中国
- 2025年07月14日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A02975-2 |
|---|---|
| 产品名称 | Anti-GOLM1 Antibody |
| 基因名 | GOLM1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 73KD |
| 免疫原 | E.coli-derived mouse Golm1/GOLPH2 recombinant protein (Position: S37-A393). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. |
| 研究类别 | 1. Kladney, R. D., Bulla, G. A., Guo, L., Mason, A. L., Tollefson, A. E., Simon, D. J., Koutoubi, Z., Fimmel, C. J. GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249: 53-65, 2000. |
| Uniprot ID | GOLM1: Q91XA2 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section IHC | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1.ICC analysis using anti- Golm1/GOLPH2 antibody (A02975-2).was detected in immersion fixed HEPA1-6 cell line . Cells were stained using the Dylight488-conjugated Anti- rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 2. Western blot analysis of anti- Golm1/GOLPH2 antibody (A02975-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Mouse stomach tissue lysates,Lane 2: Mouse lung tissue lysates,Lane 3: Mouse RAW264.7 whole cell lysates,Lane 4: Mouse Neuro-2a whole cell lysates,Lane 5: Rat stomach tissue lysates,Lane 6: Rat lung tissue lysates,Lane 7: Rat liver tissue lysates,Use rabbit anti- Golm1/GOLPH2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Golm1/GOLPH2 at approximately 73KD. The expected band size for Golm1/GOLPH2 is at 73KD.|Figure 3.Flow cytometry analysis of HEPA1-6 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 4. IHC analysis using anti- Golm1/GOLPH2 antibody (A02975-2). detected in paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- Golm1/GOLPH2 antibody (A02975-2). detected in paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-Golm1/GOLPH2 Antibody(A02975-2-100ul)
￥1960
