- ￥1960
- BOSTER
- M00374-1-100ul
- 中国
- 2025年07月15日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M00374-1 |
|---|---|
| 产品名称 | Anti-MCM2 Antibody (monoclonal, 11C4) |
| 基因名 | MCM2 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 125KD |
| 免疫原 | E.coli-derived human MCM2 recombinant protein (Position: S393-R850). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2), also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes. |
| 研究类别 | 1. Blow, J. J., Laskey, R. A. A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 332: 546-548, 1988.2. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995.3. Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V (Jul 1991). "Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle". Biochem Biophys Res Commun 177 (1): 395–400. |
| Uniprot ID | MCM2: P49736 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- MCM2 antibody (M00374-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: Monkey COS-7 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human THP-1 whole cell lysates.Use rabbit anti- MCM2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MCM2 at approximately 125KD. The expected band size for MCM2 is at 125KD.|Figure 2.IHC analysis using anti- MCM2 antibody (M00374-1).detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3.IHC analysis using anti- MCM2 antibody (M00374-1).detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.ICC analysis using anti- MCM2 antibody (M00374-1). was detected in immersion fixed HELA cell . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 5.IF analysis using anti- MCM2 antibody (M00374-1).detected in paraffin-embedded section of human tonsil tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody(green)(Catalog # BA1126) and counterstained with DAPI (blue).|Figure 6. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human HELA whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: Monkey COS-7 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human THP-1 whole cell lysates.Use rabbit anti- MCM2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for MCM2 at approximately 125KD. The expected band size for MCM2 is at 125KD.|Figure 2.IHC analysis using anti- MCM2 antibody (M00374-1).detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3.IHC analysis using anti- MCM2 antibody (M00374-1).detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.ICC analysis using anti- MCM2 antibody (M00374-1). was detected in immersion fixed HELA cell . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 5.IF analysis using anti- MCM2 antibody (M00374-1).detected in paraffin-embedded section of human tonsil tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody(green)(Catalog # BA1126) and counterstained with DAPI (blue).|Figure 6. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-MCM2 Antibody (monoclonal, 11C4)(M00374-1-100ul)
￥1960
