- ￥1960
- BOSTER
- A05107-2-100ul
- 中国
- 2025年07月14日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A05107-2 |
|---|---|
| 产品名称 | Anti-Cyt 19/As3mt Antibody |
| 基因名 | As3mt |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 44KD |
| 免疫原 | E.coli-derived mouse Cyt 19/As3mt recombinant protein (Position: M1-D346). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing. |
| 背景资料 | AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism. This gene is mapped to chromosome 10q24. |
| 研究类别 | 1. Hernandez, A., Xamena, N., Sekaran, C., Tokunaga, H., Sampayo-Reyes, A., Quinteros, D., Creus, A., Marcos, R. High arsenic metabolic efficiency in AS3MT-287Thr allele carriers. Pharmacogenet. Genomics 18: 349-355, 2008. 2. Lin, S., Shi, Q., Nix, F. B., Styblo, M., Beck, M. A., Herbin-Davis, K. M., Hall, L. L., Simeonsson, J. B., Thomas, D. J. A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol. J. Biol. Chem. 277: 10795-10803, 2002.3. Wood, T. C., Salavagionne, O. E., Mukherjee, B., Wang, L., Klumpp, A. F., Thomae, B. A., Eckloff, B. W., Schaid, D. J., Wieben, E. D., Weinshilboum, R. M. Human arsenic methyltransferase (AS3MT) pharmacogenetics: Gene resequencing and functional genomics studies. J. Biol. Chem. 281: 7364-7373, 2006. |
| Uniprot ID | As3mt: Q91WU5 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-AS3MT antibody (A05107-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse liver tissue lysate,Lane 2: mouse heart tissue lysate,Lane 3: mouse Neuro-2a whole cell lysates,?Use rabbit anti- AS3MT 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AS3MT at approximately 44KD. The expected band size for AS3MT is at 42KD.|Figure 2.Flow cytometry analysis of mouse spleen cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Cyt 19/As3mt Antibody(A05107-2-100ul)
￥1960
