- ￥1960
- BOSTER
- PB9330-100ul
- 中国
- 2025年07月13日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | PB9330 |
|---|---|
| 产品名称 | Anti-CAPN1 Antibody |
| 基因名 | CAPN1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 82KD |
| 免疫原 | E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. |
| 研究类别 | 1. Smith IJ, et al. Calpain activity is increased in skeletal muscle from gastric cancer patients with no or minimal weight loss. Muscle Nerve, 2011 Mar.2. Wang JC, et al. Proteolysis by endogenous calpain I leads to the activation of calcineurin in human heart. Clin Lab, 2012. |
| Uniprot ID | CAPN1: P07384 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- CAPN1 antibody (PB9330). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: PC-3 whole cell lysates,
Lane 5: T47D whole cell lysates,
Lane 6: A549 whole cell lysates,
Lane 7: rat testis tissue lysates,
Lane 8: mouse lung tissue lysates.
Use rabbit anti- CAPN1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CAPN1 at approximately 82KD. The expected band size for CAPN1 is at 82KD.|Figure 2. IHC analysis of Calpain1 using anti-Calpain1 antibody (PB9330).Calpain1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpain1 Antibody (PB9330) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3.ICC analysis using anti-CAPN1 antibody (PB9330) was detected in immersion fixed U20S cell line . Cells were stained using theDylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).|Figure 4.Flow cytometry analysis of PC-3 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: PC-3 whole cell lysates,
Lane 5: T47D whole cell lysates,
Lane 6: A549 whole cell lysates,
Lane 7: rat testis tissue lysates,
Lane 8: mouse lung tissue lysates.
Use rabbit anti- CAPN1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CAPN1 at approximately 82KD. The expected band size for CAPN1 is at 82KD.|Figure 2. IHC analysis of Calpain1 using anti-Calpain1 antibody (PB9330).Calpain1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpain1 Antibody (PB9330) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3.ICC analysis using anti-CAPN1 antibody (PB9330) was detected in immersion fixed U20S cell line . Cells were stained using theDylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).|Figure 4.Flow cytometry analysis of PC-3 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Calpain 1/CAPN1 Antibody(原货号PB0282)(PB9330-100ul)
￥1960
