Anti-CaV1.3/CACNA1D Antibody(原

[list_product_images]Figure 1. Western blot analysis of CAV1.3 using anti-CAV1.3 antibody (PB9329). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Brain Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV1.3 antigen affinity purified polyclonal antibody (Catalog # PB9329) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV1.3 at approximately 245KD. The expected band size for CAV1.3 is at 245KD.|Figure 2. Flow Cytometry analysis of U-87 cells using anti-CaV1.3 antibody (PB9329).Overlay histogram showing U-87 cells stained with PB9329 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaV1.3 Antibody (PB9329,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]