- ￥1960
- BOSTER
- A08388-2-100ul
- 中国
- 2025年07月13日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A08388-2 |
|---|---|
| 产品名称 | Anti-UPF3A Antibody |
| 基因名 | UPF3A |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 51KD |
| 免疫原 | E.coli-derived human UPF3A recombinant protein (Position: E4-E452). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Regulator of nonsense transcripts 3A is a protein that in humans is encoded by the UPF3A gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Several splice variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Chan, W.-K., Bhalla, A. D., Le Hir, H., Nguyen, L. S., Huang, L., Gecz, J., Wilkinson, M. F. A UPF3-mediated regulatory switch that maintains RNA surveillance. Nature Struct. Molec. Biol. 16: 747-753, 2009.2. Gross, M. B. Personal Communication. Baltimore, Md. 2/28/2011.3. Kim, V. N., Kataoka, N., Dreyfuss, G. Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. Science 293: 1832-1836, 2001. |
| Uniprot ID | UPF3A: Q9H1J1 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- UPF3A antibody (A08388-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human hela whole cell lysates,Lane 2: human hepg2 whole cell lysates,Lane 3: human HEK293 whole cell lysates,Lane 4: Rat PC-12 whole cell lysates,Lane 5: Mouse NIH3T3 whole cell lysates. Use rabbit anti- UPF3A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for UPF3A at approximately 51KD. The expected band size for UPF3A is at 51KD.|Figure 2.ICC analysis using anti- UPF3A antibody (A08388-2). was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 3.Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-UPF3A Antibody(A08388-2-100ul)
￥1960
