A synthetic peptide corresponding to a sequence at the N-terminus of human Pea3 (1-41aa MERRMKAGYLDQQVPYTFSSKSPGNGSLREALIGPLGKLMD), different from the related mouse sequence by seven amino acids.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
ETS translocation variant 4 (ETV4), also known as polyoma enhancer activator 3 (PEA3), or E1AF, is a member of the PEA3 subfamily of Ets transcription factors. It is mapped to 17q21.31. E1AF can activate the promoters of various matrix metalloproteinases, genes whose expression is associated with tumor cell invasion and metastasis, by 10 to 20 fold. Pea3 is detected in cells of epithelial and fibroblastic origin. It is also a transcriptional activator that binds to the enhancer of the adenovirus E1A gene.
研究类别
1. Davidson B, Goldberg I, Tell L, et al. (2004). "The clinical role of the PEA3 transcription factor in ovarian and breast carcinoma in effusions".°Clin. Exp. Metastasis 21 (3): 191–9.2. Higashino, F., Yoshida, K., Noumi, T., Seiki, M., Fujinaga, K. Ets-related protein E1A-F can activate three different matrix metalloproteinase gene promoters. Oncogene 10: 1461-1463, 1995. 3. Isobe M, Yamagishi F, Yoshida K, Higashino F, Fujinaga K (Feb 1996). "Assignment of the ets-related transcription factor E1A-F gene (ETV4) to human chromosome region 17q21". Genomics 28 (2): 357–9.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Figure 1. Western blot analysis of Anti-ETV4 antibody (PB0778). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat lung tissue lysates,Lane 2: HELA whole cell lysates,Lane 3: A549 whole cell lysates,Lane 4: SW620 whole cell lysates,Use rabbit Anti-ETV4 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ETV4 at approximately 68KD. The expected band size for ETV4 is at 54KD.[/list_product_images]