Anti-FMO1 Antibody(PB0621-100u

[list_product_images]Figure 1. Western blot analysis of FMO1 using anti-FMO1 antibody (PB0621). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate,Lane 2: Mouse Liver Tissue Lysate,Lane 3: Rat Kidney Tissue Lysate,Lane 4: Mouse Kidney Tissue Lysate,Lane 5: SMMC Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO1 antigen affinity purified polyclonal antibody (Catalog # PB0621) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO1 at approximately 60KD. The expected band size for FMO1 is at 60KD.[/list_product_images]