- ￥1960
- BOSTER
- M01571-1-100ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | M01571-1 |
|---|---|
| 产品名称 | Anti-AIFM1 Antibody (monoclonal, 2I5) |
| 基因名 | AIFM1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:2I5) |
| 抗体亚型 | Mouse IgG1 |
| 分子量 | 67KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human AIF(582-613aa FNRMPIARKIIKDGEQHEDLNEVAKLFNIHED), identical to the related mouse and rat sequences. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10. |
| 研究类别 | Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis (PubMed:1577597 |
| Uniprot ID | AIFM1: O95831 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates,
Lane 6: HepG2 whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates,Use rabbit Anti-AIF/AIFM1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for AIF/AIFM1 at approximately 67KD. The expected band size for AIF/AIFM1 is at 67KD.|Figure 2. Western blot analysis of Anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Mouse spleen lysates,
Lane 4: Mouse testicular tissue lysates,
Lane 5: Mouse kidney tissue lysates,
Lane 6: RAW246.7 whole cell lysates,Use rabbit Anti-AIF/AIFM1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for AIF/AIFM1 at approximately 67KD. The expected band size for AIF/AIFM1 is at 67KD.|Figure 3. IHC analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IF analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of Raji cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).|Figure 8.ICC analysis using anti- AIF/AIFM1 antibody (M01571-1). was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). [/list_product_images]
Lane 1: A549 whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: PC-3 whole cell lysates,
Lane 4: Hela whole cell lysates,
Lane 5: Caco-2 whole cell lysates,
Lane 6: HepG2 whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates,Use rabbit Anti-AIF/AIFM1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for AIF/AIFM1 at approximately 67KD. The expected band size for AIF/AIFM1 is at 67KD.|Figure 2. Western blot analysis of Anti-AIF/AIFM1 antibody (M01571-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Mouse spleen lysates,
Lane 4: Mouse testicular tissue lysates,
Lane 5: Mouse kidney tissue lysates,
Lane 6: RAW246.7 whole cell lysates,Use rabbit Anti-AIF/AIFM1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for AIF/AIFM1 at approximately 67KD. The expected band size for AIF/AIFM1 is at 67KD.|Figure 3. IHC analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IF analysis using Anti-AIF/AIFM1 antibody (M01571-1) detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue). |Figure 7. Flow cytometry analysis of Raji cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).|Figure 8.ICC analysis using anti- AIF/AIFM1 antibody (M01571-1). was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). [/list_product_images]
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Anti-AIF Antibody (monoclonal, 2I5)(M01571-1-100ul)
￥1960
