- ￥1960
- BOSTER
- BM4877-100ul
- 中国
- 2025年07月11日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BM4877 |
|---|---|
| 产品名称 | Anti-IDE Antibody |
| 基因名 | IDE |
| 抗体来源 | Rabbit |
| 克隆 | Monoclonal |
| 抗体亚型 | Rabbit |
| 分子量 | 118KD |
| 免疫原 | A synthesized peptide derived from human IDE |
| 内容 | 500 ug/ml;Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 纯化方式 | Affinity-chromatography |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| 研究类别 | 1. "Entrez Gene: IDE insulin-degrading enzyme".2. Affholter JA, Fried VA, Roth RA (Dec 1988). "Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III". Science. 242 (4884): 1415–8. |
| Uniprot ID | IDE: P14735 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:20-100 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Western blot analysis of IDE expression in HepG2 cell lysate.|Figure 2. Western blot analysis of anti- IDE antibody (BM4877). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat intestines tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse intestines tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
Use rabbit anti- IDE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IDE at approximately 118KD. The expected band size for IDE is at 118KD.[/list_product_images]
Lane 1: human HELA whole cell lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat intestines tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse intestines tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
Use rabbit anti- IDE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IDE at approximately 118KD. The expected band size for IDE is at 118KD.[/list_product_images]
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Anti-IDE/Insulysin Rabbit Monoclonal Antibody(BM4877-100ul)
￥1960
