- ￥1960
- BOSTER
- BM3876-100ul
- 中国
- 2025年07月13日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BM3876 |
|---|---|
| 产品名称 | Anti-GAPDH Antibody |
| 基因名 | GAPDH |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal |
| 抗体亚型 | Mouse IgG |
| 分子量 | 36KD |
| 免疫原 | A synthesized peptide derived from human GAPDH Mouse Monoclonal |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA |
| 纯化方式 | Affinity-chromatography |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types. |
| Uniprot ID | GAPDH: P04406 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:1000-5000 |
| Immunohistochemistry in paraffin section (IHC): | 1:20-100 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:20-100 |
| Immunocoprecipitation (IP) | 1:20 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- GAPDH antibody (BM3876). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: Mouse kidney tissue lysates,Lane 4: Mouse spleen tissue lysates,Lane 5: Mouse RAW 264.7 whole cell lysates,Lane 6: Rat brain tissue lysates,Use rabbit anti-GAPDH 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GAPDH at approximately 36KD. The expected band size for GAPDH is at 36KD.|Figure 2. IHC analysis of anti- GAPDH antibody (BM3876).detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-GAPDH Mouse Monoclonal Antibody(BM3876-100ul)
￥1960
