- ￥1960
- BOSTER
- BA4510-100ul
- 中国
- 2025年07月12日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BA4510 |
|---|---|
| 产品名称 | Anti-KLK11 Antibody |
| 基因名 | KLK11 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 31KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 11(233-250aa YTKVCKYVDWIQETMKNN). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | KLK11(KALLIKREIN 11), also called PRSS20 or TLSP, is a protein that in humans is encoded by the KLK11 gene. KLK11 is a member of the kallikrein subfamily of serine proteases, which are involved in a variety of enzymatic processes. The KLK11 gene is mapped to 19q13.41. The KLK11 gene contains 6 exons, the first of which is noncoding. KLK11 shares 48% amino acid sequence identity with mouse neuropsin, 43% identity with both human trypsin-1 and human kallikrein, and 38% identity with the mouse nerve growth factor gamma subunit. Alternate splicing of the KLK11 gene results in two transcript variants encoding two different isoforms which are differentially expressed. Western blot analysis of recombinant KLK11 suggested that the protein is secreted and posttranslationally processed. |
| 研究类别 | 1. Elliott, M. B., Irwin, D. M., Diamandis, E. P. In silico identification and Bayesian phylogenetic analysis of multiple new mammalian kallikrein gene families. Genomics 88: 591-599, 2006.2. Gan, L., Lee, I., Smith, R., Argonza-Barrett, R., Lei, H., McCuaig, J., Moss, P., Paeper, B., Wang, K. Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region. Gene 257: 119-130, 2000.3. Yoshida S, Taniguchi M, Suemoto T, Oka T, He X, Shiosaka S (Oct 1998). "cDNA cloning and expression of a novel serine protease, TLSP". |
| Uniprot ID | KLK11: Q9UBX7 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Anti-KLK11 antibody (BA4510). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: U87 tissue lysates,Lane 2: A549 tissue lysates,Lane 3: HELA tissue lysates,Lane 4: MM231 tissue lysates,Lane 5: MM453 whole cell lysates,Lane 6: COLO320 whole cell lysates,Lane 7: JURKAT whole cell lysates,Use rabbit Anti-KLK11 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for KLK11 at approximately 31KD. The expected band size for KLK11 is at 31KD.|Figure 2.IHC analysis using anti-KLK11 antibody (BA4510). detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-Kallikrein 11/KLK11 Antibody(BA4510-100ul)
￥1960
