- ￥1960
- BOSTER
- BA3803-2-100ul
- 中国
- 2025年07月14日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BA3803-2 |
|---|---|
| 产品名称 | Anti-KLK10 Antibody |
| 基因名 | KLK10 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 37KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 10(252-265aa SAQHPAVYTQICKY). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | KLK10(kallikrein-related peptidase 10) also known as kallikrein 10, PRSSL1 or NES1, belongs to the kallikrein subfamily of serine proteases, which are involved in a variety of enzymatic processes. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers.KLK10 also contains 12 conserved cysteines in its protease domain that are expected to form 6 disulfide bonds, as in other serine proteases. RT-PCR detected variable levels of KLK10 expression in nearly all of the 35 adult and fetal tissues examined. The KLK10 gene contains 6 exons, the first of which is noncoding. By fluorescence in situ hybridization, Polikoff et al.(1997) mapped the PRSSL1 gene to 19q13.3. By genomic sequence analysis, Gan et al.(2000) mapped the KLK10 gene within the KLK gene cluster on chromosome 19q13. This cluster includes 13 KLK genes and 5 pseudogenes. |
| 研究类别 | 1. Elliott, M. B., Irwin, D. M., Diamandis, E. P. In silico identification and Bayesian phylogenetic analysis of multiple new mammalian kallikrein gene families. Genomics 88: 591-599, 2006. 2. Gan, L., Lee, I., Smith, R., Argonza-Barrett, R., Lei, H., McCuaig, J., Moss, P., Paeper, B., Wang, K. Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region. Gene 257: 119-130, 2000.3. Polikoff, D., Kuo, W.-L., Cochran, J. F., Wernick, M., Kowbel, D., Myambo, K., Collins, C. C. Assignment of protease, serine-like 1 (PRSSL1) to human chromosome 19q13 by in situ hybridization and radiation hybrid mapping. Cytogenet. Cell Genet. 79: 147-148, 1997. |
| Uniprot ID | KLK10: O43240 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Anti-KLK10 antibody (BA3803-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat ovary tissue lysates,Lane 2: Rat testis tissue lysates,Lane 3: Rat spleen tissue lysates,Lane 4: Rat liver tissue lysates,Lane 5: 22RV whole cell lysates,Lane 6: SROV whole cell lysates,Lane 7: HELA whole cell lysates,Lane 8: MCF-7 whole cell lysates,Lane 9: MM231 whole cell lysates,Use rabbit Anti-KLK10 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for KLK10 at approximately 37KD. The expected band size for KLK10 is at 37KD.[/list_product_images]
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Anti-Kallikrein 10/KLK10 Antibody(BA3803-2-100ul)
￥1960
