- ￥1960
- BOSTER
- BA3670-100ul
- 中国
- 2025年07月09日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BA3670 |
|---|---|
| 产品名称 | Anti-XRCC1 Antibody |
| 基因名 | XRCC1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 80-90KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1(15-34aa QDSTHCAENLLKADTYRKWR), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | XRCC1(X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly(ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain. |
| 研究类别 | 1. Bhattacharyya, N., Banerjee, S. A novel role of XRCC1 in the functions of a DNA polymerase beta variant. Biochemistry 40: 9005-9013, 2001.2. Brookman, K. W., Tebbs, R. S., Allen, S. A., Tucker, J. D., Swiger, R. R., Lamerdin, J. E., Carrano, A. V., Thompson, L. H. Isolation and characterization of mouse Xrcc1, a DNA repair gene affecting ligation. Genomics 22: 180-188, 1994. |
| Uniprot ID | XRCC1: P18887 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- XRCC1 antibody (BA3670). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: Jurkat whole cell lysates.
Use rabbit anti- XRCC1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for XRCC1 at approximately 80-90KD. The expected band size for XRCC1 is at 69KD.|Figure 2. IHC analysis using anti- XRCC1 antibody (BA3670). detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|ICC: A549 Cell[/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: Jurkat whole cell lysates.
Use rabbit anti- XRCC1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for XRCC1 at approximately 80-90KD. The expected band size for XRCC1 is at 69KD.|Figure 2. IHC analysis using anti- XRCC1 antibody (BA3670). detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|ICC: A549 Cell[/list_product_images]
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Anti-XRCC1 Antibody(BA3670-100ul)
￥1960
