- ￥1960
- BOSTER
- BA3591-2-100ul
- 中国
- 2025年07月08日
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- 文献和实验
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BA3591-2 |
|---|---|
| 产品名称 | Anti-FCAR Antibody |
| 基因名 | FCAR |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 32KD,55KD |
| 免疫原 | A synthetic peptide corresponding to a sequence in the middle of human CD89(84-101aa EFVIDHMDANKAGRYQCQ). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | FCAR, Receptor for Fc fragment of IGA, is also known as CD89. Human Fc-alpha receptor(FCAR) is present on a number of cell types, including neutrophils, monocytes, macrophages, and eosinophils. FCAR interacts with aggregated IgAs, such as IgA coated on the surface of an invading microorganism, and mediates several immunologic defense processes such as phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. FCAR is a glycoprotein of 50 to 100 kD, with diversity on different cell types. FCAR is mapped to 19q13.4. Human COS cells transfected with FCAR cDNA bind to IgA, but not IgG. |
| 研究类别 | 1. Kremer, E. J., Kalatzis, V., Baker, E., Callen, D. F., Sutherland, G. R., Maliszewski, C. R.The gene for the human IgA Fc receptor maps to 19q13.4.Hum. Genet. 89: 107-108, 1992.2. Maliszewski, C. R., March, C. J., Schoenborn, M. A., Gimpel, S., Shen, L.Expression cloning of a human Fc receptor for IgA.J. Exp. Med. 172: 1665-1672, 1990. |
| Uniprot ID | FCAR: P24071 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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文献和实验proteases, purify the antibody of interest if possible. Otherwise just freeze 100 uL aliquots and use as needed by thawing when needed. Don't refreeze.
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
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