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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | BM0120 |
|---|---|
| 产品名称 | Anti-S100B Antibody(monoclonal, SH-B1) |
| 基因名 | S100B |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal (Clone: SH-B1) |
| 抗体亚型 | Mouse IgG1 |
| 免疫原 | Bovine brain S-100b. |
| 内容 | 200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA |
| 纯化方式 | Ascites |
| 浓度 | 200ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | S100 protein is a 21,000-Da component first isolated from brain by Moore(1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin(Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a(0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas. |
| 研究类别 | 1. Engelkamp, D.; Schafer, B. W.; Mattei, M. G.; Erne, P.; Heizmann, C. W. : Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc. Nat. Acad. Sci. 90: 6547-6551, 1993. 2. Herrera, G. A.; Turbat-Herrera, E. A.; Lott, R. L. : S-100 protein expression by primary and metastatic adenocarcinomas. Am. J. Clin. Path. 89: 168-176, 1988. |
| Uniprot ID | S100B: Human(P04271), Mouse(P50114), Rat(P04631) |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]IHC(P): Rat Brain Tissue Lysate[/list_product_images]
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Anti-S-100(beta-subunit) Antibody (monoclonal, SH-B1)(BM0120-100ul)
¥1960







