A synthetic peptide corresponding to a sequence at the C-terminus of human G-protein coupled receptor 30(275-288aa ENVFISVHLLQRTQ), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
G-protein coupled estrogen receptor 1(GPER1), also known as the GPR30 or CEPR is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. It is mapped to 7p22.3. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
研究类别
1. Pietras, R. J., Levin, E. R., Szego, C. M. Estrogen receptors and cell signaling. (Letter)Science 310: 51-52, 2005.2. Revankar, C. M., Cimino, D. F., Sklar, L. A., Arterburn, J. B., Prossnitz, E. R. A transmembrane intracellular estrogen receptor mediates rapid cell signaling. Science 307: 1625-1630, 2005. 3. Takada, Y., Kato, C., Kondo, S., Korenaga, R., Ando, J. Cloning of cDNAs encoding G protein-coupled receptor expressed in human endothelial cells exposed to fluid shear stress. Biochem. Biophys. Res. Commun. 240: 737-741, 1997.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。