Anti-CTR1(SLC31A1) Antibody(BA

[list_product_images]Figure 1. Western blot analysis of SLC31A1/CTR1 using anti-SLC31A1/CTR1 antibody (BA2421).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human CEM whole cell lysates,Lane 2: human A549 whole cell lysates， Lane 3: human SMMC whole cell lysates，Lane 4: human MCF-7 whole cell lysates， After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC31A1/CTR1 antigen affinity purified polyclonal antibody (Catalog # BA2421) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC31A1/CTR1 at approximately 35KD. The expected band size for SLC31A1/CTR1 is at 21KD.|Figure 2. IHC analysis using anti- SLC31A1 antibody (BA2421). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]