- ￥1960
- BOSTER
- BA0959-100ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | BA0959 |
|---|---|
| 产品名称 | Anti-ITGB2 Antibody |
| 基因名 | ITGB2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 90-100KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human CD18(24-58aa, ECTKFKVSSCRECIESGPGCTWCQKLNFTGPGDPD), different from the related mouse sequence by five amino acids. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The 3 alpha integrin chains associated individually with the beta-2 chain as a heterodimer have gene designations of ITGAL, ITGAM, and ITGAX, and leukocyte antigen designations of CD11A, CD11B, and CD11C, respectively. The expression of CD18 was increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. The ITGB2 gene spans approximately 40 kb and contains 16 exons and all exon/intron boundaries conform to the GT/AG splicing consensus. Furthermore, ITGB2 was constitutively clustered. Although it was expressed on the cell surface at normal levels and was capable of function following extracellular stimulation, it could not be activated via the “inside-out” signaling pathways. |
| 研究类别 | 1. Taylor, G. M.; Williams, A.; D'Souza, S. W.; Fergusson, W. D.; Donnai, D.; Fennell, J.; Harris, R. : The expression of CD18 is increased on trisomy 21 (Down syndrome) lymphoblastoid cells. Clin. Exp. Immun. 71: 324-328, 1988.2. Weitzman, J. B.; Wells, C. E.; Wright, A. H.; Clark, P. A.; Law, S. K. A. : The gene organisation of the human beta-2 integrin subunit (CD18). FEBS Lett. 294: 97-103, 1991.3. McDowall, A.; Inwald, D.; Leitinger, B.; Jones, A.; Liesner, R.; Klein, N.; Hogg, N. : A novel form of integrin dysfunction involving beta-1, beta-2, and beta-3 integrins. J. Clin. Invest. 111: 51-60, 2003. |
| Uniprot ID | ITGB2: P05107 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- ITGB2 antibody (BA0959). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- ITGB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ITGB2 at approximately 90-100KD. The expected band size for ITGB2 is at 85KD.|Figure 2. IHC analysis using anti- ITGB2 antibody (BA0959). detected in paraffin-embedded section of human intestinal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ITGB2 antibody (BA0959). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.[/list_product_images]
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- ITGB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ITGB2 at approximately 90-100KD. The expected band size for ITGB2 is at 85KD.|Figure 2. IHC analysis using anti- ITGB2 antibody (BA0959). detected in paraffin-embedded section of human intestinal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ITGB2 antibody (BA0959). detected in paraffin-embedded section of human lung cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.[/list_product_images]
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文献和实验相关实验
proteases, purify the antibody of interest if possible. Otherwise just freeze 100 uL aliquots and use as needed by thawing when needed. Don't refreeze.
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
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Anti-CD18/ITGB2 Antibody(BA0959-100ul)
￥1960
