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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | BA1689-1 |
|---|---|
| 产品名称 | Anti-PDPN Antibody |
| 基因名 | PDPN |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 36KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Podoplanin/gp36(226-238aa VVVMRKMSGRYSP). |
| 内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Podoplanin(PDPN) is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The International radiation Hybrid mapping consortium mapped the T1A gene to chromosome 1. It has got 162 amino acid protein which is a typical type I membrane glycoprotein, with an N-terminal signal sequence, a signal sequence cleavage site, a C-terminal hydrophobic stretch, and a short cytoplasmic tail. Northern blot analysis detected a 1.2-kb T1A2 transcript only in lung. It has been described as a differentiation antigen and influenza-virus receptor, and a marker of lung injury. |
| 研究类别 | 1. Zimmer G, Oeffner F, Von Messling V, Tschernig T, Groness HJ, Klenk HD, Herrler G (Sep 1999). "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium". Biochem J 341 ( Pt 2): 277–84. PMC 1220357. PMID 10393083. 2. Ma T, Yang B, Matthay MA, Verkman AS (Jul 1998). "Evidence against a role of mouse, rat, and two cloned human t1alpha isoforms as a water channel or a regulator of aquaporin-type water channels". Am J Respir Cell Mol Biol 19 (1): 143–9. PMID 9651190.3. Entrez Gene: PDPN podoplanin". |
| Uniprot ID | PDPN: Q86YL7 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Anti-PDPN antibody (BA1689-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: SMMC whole cell lysates,Lane 2: 293T whole cell lysates,Lane 3: HELA whole cell lysates,Use rabbit Anti-PDPN 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PDPN at approximately 36KD. The expected band size for PDPN is at 36KD.[/list_product_images]
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Anti-Podoplanin/gp36/PDPN Antibody(BA1689-1-100ul)
¥1960







