- ￥1960
- BOSTER
- A06453-1-100ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A06453-1 |
|---|---|
| 产品名称 | Anti-SCN4B Antibody |
| 基因名 | SCN4B |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 25KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human SCN4B (LRDLEFSDTGKYTCHVKNPKENNLQHHATIFLQ). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Sodium channel β-subunit 4, also known as SCN4B or Naβ4, is a protein that in humans is encoded by the SCN4B gene. The protein encoded by this gene is one of several sodium channel beta subunits. These subunits interact with voltage-gated alpha subunits to change sodium channel kinetics. The encoded transmembrane protein forms interchain disulfide bonds with SCN2A. Defects in this gene are a cause of long QT syndrome type 10 (LQT10). Three protein-coding and one non-coding transcript variant have been found for this gene. |
| 研究类别 | 1. Li, R.-G., Wang, Q., Xu, Y.-J., Zhang, M., Qu, X.-K., Liu, X., Fang, W.-Y., Yang, Y.-Q. Mutations of the SCN4B-encoded sodium channel beta-4 subunit in familial atrial fibrillation. Int. J. Molec. Med. 32: 144-150, 2013.2. Medeiros-Domingo, A., Kaku, T., Tester, D. J., Iturralde-Torres, P., Itty, A., Ye, B., Valdivia, C., Ueda, K., Canizales-Quinteros, S., Tusie-Luna, M. T., Makielski, J. C., Ackerman, M. J. SCN4B-encoded sodium channel beta-4 subunit in congenital long-QT syndrome. Circulation 116: 134-142, 2007. |
| Uniprot ID | SCN4B: Q8IWT1 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of SCN4B using anti-SCN4B antibody (A06453-1). Lane 1: human placenta tissue lysates,Lane 2: human U-87MG whole cell lysates,Lane 3: monkey COS-7 whole cell lysates,Lane 4: human U2OS whole cell lysates,Lane 5: human HEK293 whole cell lysates,Lane 6: human SHG-44 whole cell lysates,Lane 7: human K562 whole cell lysates (negative control),Lane 8: human HL-60 whole cell lysates (negative control). anti-SCN4B antigen affinity purified polyclonal antibody (Catalog # A06453-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SCN4B at approximately 28KD. The expected band size for SCN4B is at 25KD.|Figure 2. Western blot analysis of SCN4B using anti-SCN4B antibody (A06453-1). Lane 1: rat brain tissue lysates,Lane 2: rat heart tissue lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat kidney tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse heart tissue lysates,Lane 7: mouse spleen tissue lysates,Lane 8: mouse kidney tissue lysates,Lane 9: mouse Neuro-2a whole cell lysates. anti-SCN4B antigen affinity purified polyclonal antibody (Catalog # A06453-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SCN4B at approximately 28KD. The expected band size for SCN4B is at 25KD.|Figure 3. IHC analysis of SCN4B using anti-SCN4B antibody (A06453-1).SCN4B was detected in paraffin-embedded section of human renal cancer tissue. anti-SCN4B Antibody (A06453-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of SCN4B using anti-SCN4B antibody (A06453-1).SCN4B was detected in paraffin-embedded section of rat spleen tissue. anti-SCN4B Antibody (A06453-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. Flow Cytometry analysis of U20S cells using anti-SCN4B antibody (A06453-1).
Overlay histogram showing U20S cells stained with A06453-1 (Blue line).anti-SCN4B Antibody (A06453-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 6. Flow Cytometry analysis of SiHa cells using anti-SCN4B antibody (A06453-1).
Overlay histogram showing SiHa cells stained with A06453-1 (Blue line).anti-SCN4B Antibody (A06453-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Overlay histogram showing U20S cells stained with A06453-1 (Blue line).anti-SCN4B Antibody (A06453-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 6. Flow Cytometry analysis of SiHa cells using anti-SCN4B antibody (A06453-1).
Overlay histogram showing SiHa cells stained with A06453-1 (Blue line).anti-SCN4B Antibody (A06453-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-SCN4B Antibody(A06453-1-100ul)
￥1960
