- ￥1960
- BOSTER
- A05905-100ul
- 中国
- 2025年07月08日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A05905 |
|---|---|
| 产品名称 | Anti-BCL9L Antibody |
| 基因名 | BCL9L |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 200KD |
| 免疫原 | E.coli-derived human BCL9L recombinant protein (Position: E397-A666). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514 517 and 970 973), and one poly-Ala segment (aa 900 903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391 1426. Over aa 1009 1328, human Bcl-9 is 96% aa identical to mouse Bcl-9. |
| 研究类别 | 1. Brembeck, F. H., Schwarz-Romond, T., Bakkers, J., Wilhelm, S., Hammerschmidt, M., Birchmeier, W. Essential role of BCL9-2 in the switch between beta-catenin's adhesive and transcriptional functions. Genes Dev. 18: 2225-2230, 2004.2. Katoh, M., Katoh, M. Identification and characterization of human BCL9L gene and mouse Bcl9l gene in silico. Int. J. Molec. Med. 12: 643-649, 2003. |
| Uniprot ID | BCL9L: Q86UU0 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. IF analysis of BCL9L using anti- BCL9L antibody (A05905).
BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 2. Flow Cytometry analysis of SiHa cells using anti- BCL9L antibody (A05905).
Overlay histogram showing SiHa cells stained with A05905 (Blue line). DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human lung cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human Ovarian cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of rat kidney tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human oesophagus squama cancer tissues. anti-BCL9L Antibody (A05905) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human placenta tissues. anti-BCL9L Antibody (A05905) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of mouse kidney tissues. anti-BCL9L Antibody (A05905) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 9. IF analysis of BCL9L using anti- BCL9L antibody (A05905).
BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- BCL9L Antibody (A05905) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 10. IF analysis of BCL9L using anti- BCL9L antibody (A05905).
BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- BCL9L Antibody (A05905) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 11. Western blot analysis of BCL9L using anti-BCL9L antibody (A05905).
The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: rat testicular issue lysates,
Lane 5: rat liver issue lysates,
Lane 6: mouse testicular issue lysates,
Lane 7: mouse thymus issue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
anti-BCL9L antigen affinity purified polyclonal antibody (Catalog # A05905)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for BCL9L at approximately 200KD. The expected band size for BCL9L is at 157KD.[/list_product_images]
BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 2. Flow Cytometry analysis of SiHa cells using anti- BCL9L antibody (A05905).
Overlay histogram showing SiHa cells stained with A05905 (Blue line). DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human lung cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human Ovarian cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of rat kidney tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human oesophagus squama cancer tissues. anti-BCL9L Antibody (A05905) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of human placenta tissues. anti-BCL9L Antibody (A05905) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 8. IHC analysis of BCL9L using anti-BCL9L antibody (A05905).BCL9L was detected in paraffin-embedded section of mouse kidney tissues. anti-BCL9L Antibody (A05905) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 9. IF analysis of BCL9L using anti- BCL9L antibody (A05905).
BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- BCL9L Antibody (A05905) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 10. IF analysis of BCL9L using anti- BCL9L antibody (A05905).
BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- BCL9L Antibody (A05905) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 11. Western blot analysis of BCL9L using anti-BCL9L antibody (A05905).
The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: rat testicular issue lysates,
Lane 5: rat liver issue lysates,
Lane 6: mouse testicular issue lysates,
Lane 7: mouse thymus issue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
anti-BCL9L antigen affinity purified polyclonal antibody (Catalog # A05905)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for BCL9L at approximately 200KD. The expected band size for BCL9L is at 157KD.[/list_product_images]
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Anti-BCL9L Antibody(A05905-100ul)
￥1960
