- ￥1960
- BOSTER
- A05865-1-100ul
- 中国
- 2025年07月08日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A05865-1 |
|---|---|
| 产品名称 | Anti-GOLGA2 Antibody |
| 基因名 | GOLGA2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 130KD |
| 免疫原 | E. coli-derived human GM130 recombinant protein (Position: E796-E913). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. |
| 研究类别 | 1. Matsuki, T., Matthews, R. T., Cooper, J. A., van der Brug, M. P., Cookson, M. R., Hardy, J. A., Olson, E. C., Howell, B. W. Reelin and Stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment. Cell 143: 826-836, 2010. 2. Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 601 only, 2014. |
| Uniprot ID | GOLGA2: Q08379 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- GM130 antibody (A05865-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEK293 whole cell ly,
Lane 2: Jurkat whole cell ly,
Lane 3: Hela whole cell ly,
Lane 4: U87 whole cell ly,
Lane 5: PC-3 whole cell ly.
Use rabbit anti- GM130 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GM130 at approximately 130KD. The expected band size for GM130 is at 113KD. |Figure 2. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).GM130 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. anti-GM130 Antibody (A05865-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. | Figure 3. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).GM130 was detected in paraffin-embedded section of human placenta tissue. anti-GM130 Antibody (A05865-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).GM130 was detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).
GM130 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GM130 Antibody (A05865-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. Flow Cytometry analysis of PC-3 cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line).anti-GM130 Antibody (A05865-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Flow Cytometry analysis of U20S cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing U20S cells stained with A05865-1 (Blue line).anti-GM130 Antibody (A05865-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 8.ICC analysis using anti- GM130 Antibody (A05865-1).was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).[/list_product_images]
Lane 1: HEK293 whole cell ly,
Lane 2: Jurkat whole cell ly,
Lane 3: Hela whole cell ly,
Lane 4: U87 whole cell ly,
Lane 5: PC-3 whole cell ly.
Use rabbit anti- GM130 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GM130 at approximately 130KD. The expected band size for GM130 is at 113KD. |Figure 2. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).GM130 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. anti-GM130 Antibody (A05865-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. | Figure 3. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).GM130 was detected in paraffin-embedded section of human placenta tissue. anti-GM130 Antibody (A05865-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).GM130 was detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of GM130 using anti-GM130 antibody (A05865-1).
GM130 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GM130 Antibody (A05865-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. Flow Cytometry analysis of PC-3 cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line).anti-GM130 Antibody (A05865-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Flow Cytometry analysis of U20S cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing U20S cells stained with A05865-1 (Blue line).anti-GM130 Antibody (A05865-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 8.ICC analysis using anti- GM130 Antibody (A05865-1).was detected in immersion fixed U2OS cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).[/list_product_images]
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Anti-GM130/GOLGA2 Antibody(A05865-1-100ul)
￥1960
