- ￥1960
- BOSTER
- A05830-1-100ul
- 中国
- 2025年07月14日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A05830-1 |
|---|---|
| 产品名称 | Anti-LY9 Antibody |
| 基因名 | LY9 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence of human CD229/LY9(YKAQINQRNFEVTTEEEFTLFVYEQLQEPQVTMK). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 17q21.31. LY9 has also recently been designated CD229 (cluster of differentiation 229). LY9 belongs to the SLAM family of immunomodulatory receptors and interacts with the adaptor molecule SAP. |
| 研究类别 | 1. Sandrin MS, Henning MM, Lo MF, Baker E, Sutherland GR, McKenzie IF (Feb 1996). "Isolation and characterization of cDNA clones for Humly9: the human homologue of mouse Ly9". Immunogenetics. 2. Kingsmore SF, Souryal CA, Watson ML, Patel DD, Seldin MF (Aug 1995). "Physical and genetic linkage of the genes encoding Ly-9 and CD48 on mouse and human chromosomes 1". Immunogenetics.3. "Entrez Gene: LY9 lymphocyte antigen 9". |
| Uniprot ID | LY9: Q9HBG7 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. IHC analysis of CD229 using anti-CD229 antibody (A05830-1).
CD229 was detected in paraffin-embedded section of mouse spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 2. IHC analysis of CD229 using anti-CD229 antibody (A05830-1).
CD229 was detected in paraffin-embedded section of mouse spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of CD229 using anti-CD229 antibody (A05830-1).
CD229 was detected in paraffin-embedded section of rat spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of CD229 using anti-CD229 antibody (A05830-1).
CD229 was detected in paraffin-embedded section of rat spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. Flow Cytometry analysis of Jurkat cells using anti- CD229 antibody (A05830-1).
Overlay histogram showing Jurkat cells stained with A05830-1 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 6. Flow Cytometry analysis of Daudi cells using anti- CD229 antibody (A05830-1).
Overlay histogram showing Daudi cells stained with A05830-1 (Blue line) DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Flow Cytometry analysis of U937 cells using anti- CD229 antibody (A05830-1).
Overlay histogram showing U937 cells stained with A05830-1 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
CD229 was detected in paraffin-embedded section of mouse spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 2. IHC analysis of CD229 using anti-CD229 antibody (A05830-1).
CD229 was detected in paraffin-embedded section of mouse spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of CD229 using anti-CD229 antibody (A05830-1).
CD229 was detected in paraffin-embedded section of rat spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of CD229 using anti-CD229 antibody (A05830-1).
CD229 was detected in paraffin-embedded section of rat spleen tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. Flow Cytometry analysis of Jurkat cells using anti- CD229 antibody (A05830-1).
Overlay histogram showing Jurkat cells stained with A05830-1 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 6. Flow Cytometry analysis of Daudi cells using anti- CD229 antibody (A05830-1).
Overlay histogram showing Daudi cells stained with A05830-1 (Blue line) DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Flow Cytometry analysis of U937 cells using anti- CD229 antibody (A05830-1).
Overlay histogram showing U937 cells stained with A05830-1 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-CD229/LY9 Antibody(A05830-1-100ul)
￥1960
