- ￥1960
- BOSTER
- A04796-100ul
- 中国
- 2025年07月13日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A04796 |
|---|---|
| 产品名称 | Anti-ANXA3 Antibody |
| 基因名 | ANXA3 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 36KD |
| 免疫原 | E.coli-derived human Annexin A3/ANXA3 recombinant protein (Position: D23-D323). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Annexin A3 is a protein that in humans is encoded by the Annexin A3 gene. The Annexin A3 gene contains 13 exons and spans 58 kb of genomic DNA. The Annexin A3 gene is mapped to 4q21. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. |
| 研究类别 | 1. Bianchi C, et al. Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3. Am J Pathol, 2010 Apr.2. Jung EJ, et al. Decreased annexin A3 expression correlates with tumor progression in papillary thyroid cancer. Proteomics Clin Appl, 2010 May.3. Yan X, et al. Increased expression of annexin A3 is a mechanism of platinum resistance in ovarian cancer. Cancer Res, 2010 Feb 15. |
| Uniprot ID | ANXA3: P12429 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of ANXA3 using anti-ANXA3 antibody (A04796). Lane 1: rat lung tissue lysates,Lane 2: rat ovary tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: mouse ovary tissue lysates. anti-ANXA3 antigen affinity purified polyclonal antibody (Catalog # A04796)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for ANXA3 at approximately 36KD. The expected band size for ANXA3 is at 36KD.|Figure 2. IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).ANXA3 was detected in paraffin-embedded section of human intestinal cancer tissue. anti-ANXA3 Antibody (A04796) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).ANXA3 was detected in paraffin-embedded section of human lung cancer tissue. anti-ANXA3 Antibody (A04796) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).ANXA3 was detected in paraffin-embedded section of mouse kidney tissue. anti-ANXA3 Antibody (A04796) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).ANXA3 was detected in paraffin-embedded section of human mammary cancer tissue. anti-ANXA3 Antibody (A04796) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of ANXA3 using anti-ANXA3 antibody (A04796).ANXA3 was detected in paraffin-embedded section of rat kidney tissue. anti-ANXA3 Antibody (A04796) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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Anti-Annexin A3/ANXA3 Antibody(A04796-100ul)
￥1960
