- ￥1960
- BOSTER
- A04457-3-100ul
- 中国
- 2025年07月14日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A04457-3 |
|---|---|
| 产品名称 | Anti-RNF31 Antibody |
| 基因名 | RNF31 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 120KD |
| 免疫原 | E.coli-derived human RNF31/HOIP recombinant protein (Position: R970-K1072). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | RING finger protein 31 is a protein that in humans is encoded by the RNF31 gene. It is mapped to 14q12. The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Ikeda, F., Deribe, Y. L., Skanland, S. S., Stieglitz, B., Grabbe, C., Franz-Wachtel, M., van Wijk, S. J. L., Goswami, P., Nagy, V., Terzic, J., Tokunaga, F., Androulidaki, A., Nakagawa, T., Pasparakis, M., Iwai, K., Sundberg, J. P., Schaefer, L., Rittinger, K., Macek, B., Dikic, I. SHARPIN forms a linear ubiquitin ligase complex regulating NF-kappa-B activity and apoptosis.Nature 471: 637-641, 2011. 2. Thompson, H. G. R., Harris, J. W., Lin, L., Brody, J. P. Identification of the protein Zibra, its genomic organization, regulation, and expression in breast cancer cells. Exp. Cell Res. 295: 448-459, 2004. |
| Uniprot ID | RNF31: Q96EP0 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of RNF31 using anti-RNF31 antibody (A04457-3). Lane 1: human placenta tissues lysatesLane 2: human HepG2 whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: human A549 whole cell lysatesLane 5: human U2OS whole cell lysatesLane 6: human Caco-2 whole cell lysatesLane 7: human Hela whole cell lysates anti-RNF31 antigen affinity purified polyclonal antibody (Catalog # A04457-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for RNF31 at approximately 120KD. The expected band size for RNF31 is at 120KD.[/list_product_images]
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Anti-RNF31/HOIP Antibody(A04457-3-100ul)
￥1960
