- ￥1960
- BOSTER
- A04094-1-100ul
- 中国
- 2025年07月11日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A04094-1 |
|---|---|
| 产品名称 | Anti-OLFM4 Antibody |
| 基因名 | OLFM4 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 57KD |
| 免疫原 | E.coli-derived human OLFM4 recombinant protein (Position: D91-Q510). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Olfactomedin 4 is a protein that in humans is encoded by the OLFM4 gene. This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. |
| 研究类别 | 1. Dassen H, Punyadeera C, Delvoux B, Schulkens I, Marchetti C, Kamps R, Klomp J, Dijcks F, de Goeij A, D'Hooghe T, Kyama C, Ederveen A, Dunselman G, Groothuis P, Romano A (2010). "Olfactomedin-4 regulation by estrogen in the human endometrium requires epidermal growth factor signaling". Am. J. Pathol. 177 (5): 2495–508.2. van der Flier LG, Haegebarth A, Stange DE, van de Wetering M, Clevers H (2009). "OLFM4 is a robust marker for stem cells in human intestine and marks a subset of colorectal cancer cells".Gastroenterology. 137 (1): 15–7. |
| Uniprot ID | OLFM4: Q6UX06 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of OLMF4 using anti-OLMF4 antibody (A04094-1). Lane 1: human placenta tissue lysatesLane 2: human U2OS whole cell lysatesLane 3: human U-87MG whole cell lysatesLane 4: human HL-60 whole cell lysatesLane 5: human K562 whole cell lysatesLane 6: human THP-1 whole cell lysates anti-OLMF4 antigen affinity purified polyclonal antibody (Catalog # A04094-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for OLMF4 at approximately 75KD. The expected band size for OLMF4 is at 57KD.|Figure 2. Western blot analysis of OLMF4 using anti-OLMF4 antibody (A04094-1). Lane 1: rat spleen tissue lysatesLane 2: rat kidney tissue lysatesLane 3: mouse kidney tissue lysatesLane 4: mouse lung tissue lysatesLane 5: mouse SP20 whole cell lysates anti-OLMF4 antigen affinity purified polyclonal antibody (Catalog # A04094-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for OLMF4 at approximately 75KD. The expected band size for OLMF4 is at 57KD.|Figure 3. IHC analysis of OLMF4 using anti-OLMF4 antibody (A04094-1).OLMF4 was detected in paraffin-embedded section of human colon cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of OLMF4 using anti-OLMF4 antibody (A04094-1).OLMF4 was detected in paraffin-embedded section of human rectal cancer tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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Anti-OLFM4 Antibody(A04094-1-100ul)
￥1960
