- ￥1960
- BOSTER
- A07981-1-100ul
- 中国
- 2025年07月11日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A07981-1 |
|---|---|
| 产品名称 | Anti-HSPB6 Antibody |
| 基因名 | HSPB6 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 17KD |
| 免疫原 | E. coli-derived human Hsp20 recombinant protein (Position: H73-L123). Human Hsp20 shares 88.2% and 92.2% amino acid (aa) sequence identity with mouse and rat Hsp20, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Heat shock protein beta-6 is a protein that in humans is encoded by the HSPB6 gene. HSPB6, also known as hsp20, is a 17-kDa member of the small heat shock family of proteins. It is expressed in multiple tissues; however, HSPB6 is most highly and constitutively expressed in different types of muscle including vascular, airway, colonic, bladder, uterine smooth muscle, cardiac muscle and skeletal muscle. HSPB6 has specific functions for vasodilation, platelet function, and insulin resistance and in smooth and cardiac muscle. |
| 研究类别 | 1. "Entrez Gene: HSPB6 heat shock protein, alpha-crystallin-related, B6".2. Bukach OV, Seit-Nebi AS, Marston SB, Gusev NB (Jan 2004). "Some properties of human small heat shock protein Hsp20 (HspB6)". Eur J Biochem. 271 (2): 291–302.3. Dreiza CM, Komalavilas P, Furnish EJ, Flynn CR, Sheller MR, Smoke CC, Lopes LB, Brophy CM (Jan 2010)."The small heat shock protein, HSPB6, in muscle function and disease". Cell Stress Chaperones. 15 (1): 1–11. |
| Uniprot ID | HSPB6: O14558 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-HSPB6 antibody (A07981-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat cardiac muscle tissue lysate,Lane 2: rat skeletal muscle tissue lysate,Lane 3: rat testis tissue lysate,Lane 4: mouse testis tissue lysate,Lane 5: human A431 whole Cell lysate.Use rabbit anti- HSPB6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSPB6 at approximately 17KD. The expected band size for HSPB6 is at 17KD.|Figure 2.IHC analysis using anti- HSPB6 antibody (A07981-1). detected in paraffin-embedded section of human ?skeletal?muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3.IHC analysis using anti- HSPB6 antibody (A07981-1). detected in paraffin-embedded section of human ?skeletal?muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.IHC analysis using anti- HSPB6 antibody (A07981-1). detected in paraffin-embedded section of human ?breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5.IHC analysis using anti- HSPB6 antibody (A07981-1). detected in paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6.IHC analysis using anti- HSPB6 antibody (A07981-1). detected in paraffin-embedded section of mouse heart tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 7.IHC analysis using anti- HSPB6 antibody (A07981-1). detected in paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 8.IHC analysis using anti- HSPB6 antibody (A07981-1). detected in paraffin-embedded section of rat heart tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 9.ICC analysis using anti-HSPB6 antibody (A07981-1) was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue). [/list_product_images]
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Anti-Hsp20/HSPB6 Antibody(A07981-1-100ul)
￥1960
