- ￥1960
- BOSTER
- A03222-1-100ul
- 中国
- 2025年07月14日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A03222-1 |
|---|---|
| 产品名称 | Anti-THPO Antibody |
| 基因名 | Thpo |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 38KD |
| 免疫原 | E. coli-derived mouse Thrombopoietin recombinant protein (Position: S22-H259). Mouse Thrombopoietin shares 79.3% and 92.9% amino acid (aa) sequence identity with human and rat Thrombopoietin, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a protein that in humans is encoded by the THPO gene. Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. |
| 研究类别 | 1. "Entrez Gene: THPO thrombopoietin (myeloproliferative leukemia virus oncogene ligand, megakaryocyte growth and development factor)".2. Kaushansky K (2006). "Lineage-specific hematopoietic growth factors". N. Engl. J. Med. 354 (19): 2034–45. |
| Uniprot ID | Thpo: P40226 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. -Thrombopoietin antigen affinity purified polyclonal antibody (Catalog # A03222-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD.|Figure 2. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).Thrombopoietin was detected in paraffin-embedded section of mouse liver tissues. anti- Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).Thrombopoietin was detected in paraffin-embedded section of mouse spleen tissues. anti- Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).Thrombopoietin was detected in paraffin-embedded section of mouse kidney tissues. anti- Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).Thrombopoietin was detected in paraffin-embedded section of rat spleen tissues. anti- Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (A03222-1).Thrombopoietin was detected in paraffin-embedded section of rat kidney tissues. anti- Thrombopoietin Antibody (A03222-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-Thrombopoietin/Thpo Antibody(A03222-1-100ul)
￥1960
