- ￥1960
- BOSTER
- A02828-2-100ul
- 中国
- 2025年07月08日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A02828-2 |
|---|---|
| 产品名称 | Anti-KRT20 Antibody |
| 基因名 | Krt20 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 48KD |
| 免疫原 | E.coli-derived mouse Cytokeratin 20/Krt20 recombinant protein (Position: R29-V431). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. This gene encodes a member of the keratin protein family and is found within a cluster of cytokeratin genes on chromosome 11. Keratins are cytoskeletal proteins that are preferentially expressed in epithelial cells. The encoded protein may help maintain intermediate filament organization in intestinal epithelium. Phosphorylation of this protein may also influence mucin secretion in the small intestine. |
| 研究类别 | 1. Calnek, D., Quaroni, A. Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation. Differentiation 53: 95-104, 1993.2. Moll, R., Schiller, D. L., Franke, W. W. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell Biol. 111: 567-580, 1990.3. Rogers, M. A., Langbein, L., Winter, H., Ehmann, C., Praetzel, S., Korn, B., Schweizer, J. Characterization of a cluster of human high/ultrahigh sulfur keratin-associated protein genes embedded in the type I keratin gene domain on chromosome 17q12-21. J. Biol. Chem. 276: 19440-19451, 2001. |
| Uniprot ID | Krt20: Q9D312 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF) | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of Krt20 using anti-Krt20 antibody (A02828-2).
Lane 1: human Caco-2 whole cell lysates,
Lane 2: mouse small intestine tissue lysates.
anti-Krt20 antigen affinity purified polyclonal antibody (Catalog # A02828-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Krt20 at approximately 48KD. The expected band size for Krt20 is at 48KD.|Figure 2. IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2).
Krt20 was detected in paraffin-embedded section of human appendix tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2).
Krt20 was detected in paraffin-embedded section of rat intestine tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2).
Krt20 was detected in paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. Flow Cytometry analysis of CACO-2 cells using anti-Krt20 antibody (A02828-2).
Overlay histogram showing CACO-2 cells stained with A02828-2 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 6. Flow Cytometry analysis of Hela cells using anti-Krt20 antibody (A02828-2).
Overlay histogram showing Hela cells stained with A02828-2 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Flow Cytometry analysis of RH35 cells using anti-Krt20 antibody (A02828-2).
Overlay histogram showing RH35 cells stained with A02828-2 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 8. Immunofluorescence assay analysis of A431 cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.[/list_product_images]
Lane 1: human Caco-2 whole cell lysates,
Lane 2: mouse small intestine tissue lysates.
anti-Krt20 antigen affinity purified polyclonal antibody (Catalog # A02828-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Krt20 at approximately 48KD. The expected band size for Krt20 is at 48KD.|Figure 2. IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2).
Krt20 was detected in paraffin-embedded section of human appendix tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2).
Krt20 was detected in paraffin-embedded section of rat intestine tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis of Krt20 using anti-Krt20 antibody (A02828-2).
Krt20 was detected in paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. Flow Cytometry analysis of CACO-2 cells using anti-Krt20 antibody (A02828-2).
Overlay histogram showing CACO-2 cells stained with A02828-2 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 6. Flow Cytometry analysis of Hela cells using anti-Krt20 antibody (A02828-2).
Overlay histogram showing Hela cells stained with A02828-2 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 7. Flow Cytometry analysis of RH35 cells using anti-Krt20 antibody (A02828-2).
Overlay histogram showing RH35 cells stained with A02828-2 (Blue line).DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 8. Immunofluorescence assay analysis of A431 cell at a dilution of 1:50 dylight 488 goat anti-rabbit IgG(green) blue Pseudocolor blue=DAPI.[/list_product_images]
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Anti-Cytokeratin 20/Krt20 Antibody(A02828-2-100ul)
￥1960
