- ￥1960
- BOSTER
- A02721-3-100ul
- 中国
- 2025年07月01日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A02721-3 |
|---|---|
| 产品名称 | Anti-APRT Antibody |
| 基因名 | APRT |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 20KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of mouse APRT(RAEVVECVSLVELTSLKGRERL). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Adenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Silva CH, Silva M, Iulek J, Thiemann OH (Jun 2008). "Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism". Journal of Biomolecular Structure & Dynamics. 25 (6): 589–97.2. Sarver AE, Wang CC (Oct 2002). "The adenine phosphoribosyltransferase from Giardia lamblia has a unique reaction mechanism and unusual substrate binding properties". The Journal of Biological Chemistry. 277(42): 39973–80.3. Valaperta R, Rizzo V, Lombardi F, Verdelli C, Piccoli M, Ghiroldi A, Creo P, Colombo A, Valisi M, Margiotta E, Panella R, Costa E (1 July 2014). "Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation". BMC Nephrology. 15: 102. |
| Uniprot ID | APRT: P08030 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of APRT using anti-APRT antibody (A02721-3). Lane 1: human T-47D whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human Caco-2 whole cell lysates. anti-APRT antigen affinity purified polyclonal antibody (Catalog # A02721-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for APRT at approximately 20KD. The expected band size for APRT is at 20KD.|Figure 2. IHC analysis of APRT using anti-APRT antibody (A02721-3).APRT was detected in paraffin-embedded section of human intestinal cancer tissue. anti-APRT Antibody (A02721-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of APRT using anti-APRT antibody (A02721-3).APRT was detected in paraffin-embedded section of human intestinal cancer tissue. anti-APRT Antibody (A02721-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of APRT using anti-APRT antibody (A02721-3).APRT was detected in paraffin-embedded section of human intestinal cancer tissue. anti-APRT Antibody (A02721-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of APRT using anti-APRT antibody (A02721-3).APRT was detected in paraffin-embedded section of human mammary cancer tissue. anti-APRT Antibody (A02721-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of APRT using anti-APRT antibody (A02721-3).APRT was detected in paraffin-embedded section of human mammary cancer tissue. anti-APRT Antibody (A02721-3) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. Western blot analysis of APRT using anti- APRT antibody (A02721-3).
Lane 1: mouse RAW246.7 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat liver tissue lysates,
anti- APRT antigen affinity purified polyclonal antibody (Catalog # A02721-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for APRT at approximately 20KD. The expected band size for APRT is at 20KD.[/list_product_images]
Lane 1: mouse RAW246.7 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat liver tissue lysates,
anti- APRT antigen affinity purified polyclonal antibody (Catalog # A02721-3)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for APRT at approximately 20KD. The expected band size for APRT is at 20KD.[/list_product_images]
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Anti-APRT Antibody(A02721-3-100ul)
￥1960
