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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
100ul
产品概况
| 货号 | A02701 |
|---|---|
| 产品名称 | Anti-TRPC5 Antibody |
| 基因名 | TRPC5 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 111KD |
| 免疫原 | E.coli-derived human TRPC5 recombinant protein (Position: L378-L973). |
| 内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Short transient receptor potential channel 5 (TrpC5), also known as TRP-5, is a protein that in humans is encoded by the TRPC5 gene. TRPC5 is subtype of the TRPC family of mammalian transient receptor potential ion channels. It is mapped to Xq23. The predicted 973-amino acid TRPC5 protein has a calculated molecular mass of 111.5 kD. It contains the characteristic 8 predicted transmembrane domains (TM1 through TM8), including a pore region (TM7) between TM6 and TM8. TRPC5 is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel, and it is a candidate for the regulation of calcium waves. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. |
| 研究类别 | 1. "Entrez Gene: transient receptor potential cation channel".2. Greka, A., Navarro, B., Oancea, E., Duggan, A., Clapham, D. E. TRPC5 is a regulator of hippocampal neurite length and growth cone morphology. Nature Neurosci. 6: 837-845, 2003.3. Sossey-Alaoui, K., Lyon, J. A., Jones, L., Abidi, F. E., Hartung, A. J., Hane, B., Schwartz, C. E., Stevenson, R. E., Srivastava, A. K. Molecular cloning and characterization of TRPC5 (HTRP5), the human homologue of a mouse brain receptor-activated capacitative Ca(2+) entry channel. Genomics 60: 330-340, 1999. |
| Uniprot ID | TRPC5: Q9UL62 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| (ELISA): | 1:100-1000 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of TRPC5 using anti-TRPC5 antibody (A02701).
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates.
anti-TRPC5 antigen affinity purified polyclonal antibody (Catalog # A02701)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for TRPC5 at approximately 111KD. The expected band size for TRPC5 is at 111KD.[/list_product_images]
Lane 1: human placenta tissue lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates.
anti-TRPC5 antigen affinity purified polyclonal antibody (Catalog # A02701)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for TRPC5 at approximately 111KD. The expected band size for TRPC5 is at 111KD.[/list_product_images]
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Anti-TRPC5 Antibody(A02701-100ul)
¥1960








