- ￥1960
- BOSTER
- A02554-1-100ul
- 中国
- 2025年07月09日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A02554-1 |
|---|---|
| 产品名称 | Anti-TNFSF4 Antibody |
| 基因名 | Tnfsf4 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 22KD |
| 免疫原 | E. coli-derived mouse CD252 recombinant protein (Position: Q49-L198). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | OX40L(TNFSF4) is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Graham, D. S. C., Graham, R. R., Manku, H., Wong, A. K., Whittaker, J. C., Gaffney, P. M., Moser, K. L., Rioux, J. D., Altshuler, D., Behrens, T. W., Vyse, T. J. Polymorphism at the TNF superfamily gene TNFSF4 confers susceptibility to systemic lupus erythematosus. Nature Genet. 40: 83-89, 2008.2. Koch, W., Hoppmann, P., Mueller, J. C., Schomig, A., Kastrati, A. Lack of support for association between common variation in TNFSF4 and myocardial infarction in a German population. (Letter) Nature Genet. 40: 1386-1387, 2008. |
| Uniprot ID | Tnfsf4: P43488 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| ELISA(Cap): | 1:50-1:200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of CD252 using anti-CD252 antibody (A02554-1). Lane 1: recombinant mouse CD252 protein 1ng. anti-CD252 antigen affinity purified polyclonal antibody (Catalog # A02554-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for CD252 at approximately 21-25KD. The expected band size for CD252 is at 18KD.|Figure 2. IHC analysis of CD252 using anti-CD252 antibody (A02554-1).CD252 was detected in paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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Anti-CD252/Tnfsf4 Antibody(A02554-1-100ul)
￥1960
