- ￥1960
- BOSTER
- A01378-100ul
- 中国
- 2025年07月16日
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- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A01378 |
|---|---|
| 产品名称 | Anti-POSTN Antibody |
| 基因名 | Postn |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 93KD |
| 免疫原 | E. coli-derived rat Periostin recombinant protein (Position: K577-Q810). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. It was found that purified recombinant PN supported adhesion of ovarian epithelial cells. And periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. It was showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle. |
| 研究类别 | 1. Gillan, L., Matei, D., Fishman, D. A., Gerbin, C. S., Karlan, B. Y., Chang, D. D. Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha-V-beta-3 and alpha-V-beta-5 integrins and promotes cell motility. Cancer Res. 62: 5358-5364, 2002. 2. Kuhn, B., del Monte, F., Hajjar, R. J., Chang, Y.-S., Lebeche, D., Arab, S., Keating, M. T. Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair. Nature Med. 13: 962-969, 2007.3. Shao, R., Bao, S., Bai, X., Blanchette, C., Anderson, R. M., Dang, T., Gishizky, M. L., Marks, J. R., Wang, X.-F. Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression. Molec. Cell. Biol. 24: 3992-4003, 2004. |
| Uniprot ID | Postn: D3ZAF5 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Periostin using anti-Periostin antibody (A01378). Lane 1: rat gaster tissue lysates, Lane 2: rat lung tissue lysates,Lane 3: rat NRK whole cell lysates,Lane 4: mouse gaster tissue lysates,Lane 5: mouse intestine tissue lysates,Lane 6: mouse lung tissue lysates. anti-Periostin antigen affinity purified polyclonal antibody (Catalog # A01378)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for Periostin at approximately 93KD. The expected band size for Periostin is at 93KD.|Figure 2. IHC analysis of Periostin using anti-Periostin antibody (A01378).Periostin was detected in paraffin-embedded section of mouse gaster tissue . anti-Periostin Antibody (A01378) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of Periostin using anti-Periostin antibody (A01378).Periostin was detected in paraffin-embedded section of mouse lung tissue. anti-Periostin Antibody (A01378) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of Periostin using anti-Periostin antibody (A01378).Periostin was detected in paraffin-embedded section of mouse small intestine tissue. anti-Periostin Antibody (A01378) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. IHC analysis of Periostin using anti-Periostin antibody (A01378).Periostin was detected in paraffin-embedded section of rat gaster tissue . anti-Periostin Antibody (A01378) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 6. IHC analysis of Periostin using anti-Periostin antibody (A01378).Periostin was detected in paraffin-embedded section of rat lung tissue. anti-Periostin Antibody (A01378) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 7. IHC analysis of Periostin using anti-Periostin antibody (A01378).Periostin was detected in paraffin-embedded section of rat small intestine tissue. anti-Periostin Antibody (A01378) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. [/list_product_images]
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Anti-Periostin/Postn Antibody(A01378-100ul)
￥1960
