- ￥1960
- BOSTER
- A00340-1-100ul
- 中国
- 2025年07月16日
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大量
- 供应商：
广州威佳科技有限公司
- 规格：
100ul
产品概况
| 货号 | A00340-1 |
|---|---|
| 产品名称 | Anti-NFATC1 Antibody |
| 基因名 | NFATC1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 101KD |
| 免疫原 | E. coli-derived human NFAT2 recombinant protein (Position: Q589-K652). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | NFATC1(Nuclear factor of activated T-cells, cytoplasmic 1), also known as NFATC or NFAT2, is a protein that in humans is encoded by the NFATC1 gene. And it is also a component of the nuclear factor of activated T cells DNA-binding transcription complex. The NFATC1 gene is mapped on 18q23. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. NFATC1 was not detected in brain, liver, or kidney. NFATC1 is an inducible nuclear component which functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. The solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the 12-bp oligonucleotide duplex containing the ARRE2 DNA site from the IL2 promoter. Nfatc1 was expressed exclusively in mouse hair follicle stem cells, and using gain- and loss-of-function approaches, and Nfatc1 inhibited stem cell activation. |
| 研究类别 | 1. Aramburu, J., Garcia-Cozar, F., Raghavan, A., Okamura, H., Rao, A., Hogan, P. G. Selective inhibition of NFAT activation by a peptide spanning the calcineurin targeting site of NFAT. Molec. Cell 1: 627-637, 1998.2. Arron, J. R., Winslow, M. M., Polleri, A., Chang, C.-P., Wu, H., Gao, X., Neilson, J. R., Chen, L., Heit, J. J., Kim, S. K., Yamasaki, N., Miyakawa, T., Francke, U., Graef, I. A., Crabtree, G. R. NFAT dysregulation by increased dosage of DSCR1 and DYRK1A on chromosome 21. Nature 441: 595-600, 2006.3. Northrop JP, Ho SN, Chen L, Thomas DJ, Timmerman LA, Nolan GP, Admon A, Crabtree GR (Jul 1994). "NF-AT components define a family of transcription factors targeted in T-cell activation". Nature 369 (6480): 497–502. |
| Uniprot ID | NFATC1: O95644 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of NFAT2 using anti-NFAT2 antibody (A00340-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates,Lane 2: human 22RV1 whole cell lysates. probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD.|Figure 2. Flow Cytometry analysis of Hela cells using anti-NFATC1 antibody (A00340-1).Overlay histogram showing Hela cells stained with A00340-1 (Blue line). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. Flow Cytometry analysis of THP-1 cells using anti-NFATC1 antibody (A00340-1).Overlay histogram showing THP-1 cells stained with A00340-1 (Blue line). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 4. Flow Cytometry analysis of MCF-7 cells using anti-NFATC1 antibody (A00340-1).Overlay histogram showing MCF-7 cells stained with A00340-1 (Blue line). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 5. Flow Cytometry analysis of Jurkat cells using anti-NFATC1 antibody (A00340-1).Overlay histogram showing Jurkat cells stained with A00340-1 (Blue line)..DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-NFAT2/NFATC1 Antibody(A00340-1-100ul)
￥1960
