- ￥1180
- BOSTER
- A01718-50ul
- 中国
- 2025年07月16日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01718 |
|---|---|
| 产品名称 | Anti-ALPP Antibody |
| 基因名 | ALPP |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 70KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human ALPP (403-433aa KARDRKAYTVLLYGNGPGYVLKDGARPDVTE). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. |
| 研究类别 | 1. "Entrez Gene: ALPP alkaline phosphatase, placental (Regan isozyme)".2. Henthorn PS, Knoll BJ, Raducha M, Rothblum KN, Slaughter C, Weiss M, Lafferty MA, Fischer T, Harris H (Sep 1986). "Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids". Proc Natl Acad Sci U S A. 83 (15): 5597–601.3. Kam W, Clauser E, Kim YS, Kan YW, Rutter WJ (Feb 1986). "Cloning, sequencing, and chromosomal localization of human term placental alkaline phosphatase cDNA". Proc Natl Acad Sci U S A. 82 (24): 8715–9. |
| Uniprot ID | ALPP: P05187 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- ALPP antibody (A01718). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human placenta tissue lysates.
Use rabbit anti- ALPP 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ALPP at approximately 70KD. The expected band size for ALPP is at 58KD.|Figure 2. IHC analysis of ALPP using anti-ALPP antibody (A01718).ALPP was detected in paraffin-embedded section of human placenta tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. ICC analysis using anti- ALPP antibody (A01718).was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 4. IF analysis of ALPP using anti-ALPP antibody (A01718).
ALPP was detected in a paraffin-embedded section of human placenta tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 5.Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human placenta tissue lysates,
Lane 2: human placenta tissue lysates.
Use rabbit anti- ALPP 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ALPP at approximately 70KD. The expected band size for ALPP is at 58KD.|Figure 2. IHC analysis of ALPP using anti-ALPP antibody (A01718).ALPP was detected in paraffin-embedded section of human placenta tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. ICC analysis using anti- ALPP antibody (A01718).was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 4. IF analysis of ALPP using anti-ALPP antibody (A01718).
ALPP was detected in a paraffin-embedded section of human placenta tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 5.Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody(A01718-50ul)
￥1180
