- ￥1180
- BOSTER
- A01281-3-50ul
- 中国
- 2025年07月14日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01281-3 |
|---|---|
| 产品名称 | Anti-CD81 Antibody |
| 基因名 | CD81 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 22KD |
| 免疫原 | E. coli-derived mouse TAPA1 recombinant protein (Position: K116-K201). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing. |
| 背景资料 | CD81 molecule, also known as CD81 (Cluster of Differentiation 81), is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Andria ML, Hsieh CL, Oren R, Francke U, Levy S (August 1991)."Genomic organization and chromosomal localization of the TAPA-1 gene".Journal of Immunology. 147 (3): 1030–6.2. Levy S, Todd SC, Maecker HT (1998). "CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system.".Annu Rev Immunol. 16: 89–109. |
| Uniprot ID | CD81: P35762 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Direct ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-CD81 antibody (A01281-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse ovary tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD81 antigen affinity purified polyclonal antibody (A01281-3) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD81 at approximately 22 kDa. The expected band size for CD81 is at 26 kDa.|Figure 2. IHC analysis of CD81 using anti-CD81 antibody (A01281-3).
CD81 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IF analysis of CD81 using anti-CD81 antibody (A01281-3).
CD81 was detected in a paraffin-embedded section of mouse liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 4. IF analysis of CD81 using anti-CD81 antibody (A01281-3).
CD81 was detected in a paraffin-embedded section of rat liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). [/list_product_images]
Lane 1: rat brain tissue lysates,
Lane 2: mouse ovary tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD81 antigen affinity purified polyclonal antibody (A01281-3) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD81 at approximately 22 kDa. The expected band size for CD81 is at 26 kDa.|Figure 2. IHC analysis of CD81 using anti-CD81 antibody (A01281-3).
CD81 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.|Figure 3. IF analysis of CD81 using anti-CD81 antibody (A01281-3).
CD81 was detected in a paraffin-embedded section of mouse liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 4. IF analysis of CD81 using anti-CD81 antibody (A01281-3).
CD81 was detected in a paraffin-embedded section of rat liver tissue. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). [/list_product_images]
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Anti-TAPA1/Cd81 Antibody(A01281-3-50ul)
￥1180
