- ￥1180
- BOSTER
- A08765-1-50ul
- 中国
- 2025年07月07日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A08765-1 |
|---|---|
| 产品名称 | Anti-RPB5/POLR2E Antibody |
| 基因名 | POLR2E |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 23KD |
| 免疫原 | E.coli-derived human RPB5/POLR2E recombinant protein (Position: Q95-Q210). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | DNA-directed RNA polymerases I, II, and III subunit RPABC1 is a protein that in humans is encoded by the POLR2E gene. This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. |
| 研究类别 | 1. Acker, J., Mattei, M.-G., Wintzerith, M., Roeckel, N., Depetris, D., Vigneron, M., Kedinger, C. Chromosomal localization of human RNA polymerase II subunit genes. Genomics 20: 496-499, 1994. 2. Cramer, P., Bushnell, D. A., Fu, J., Gnatt, A. L., Maier-Davis, B., Thompson, N. E., Burgess, R. R., Edwards, A. M., David, P. R., Kornberg, R. D. Architecture of RNA polymerase II and implications for the transcription mechanism. Science 288: 640-648, 2000. |
| Uniprot ID | POLR2E: P19388 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-RPB5/POLR2E antibody (A08765-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPB5/POLR2E antigen affinity purified polyclonal antibody (A08765-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RPB5/POLR2E at approximately 23 kDa. The expected band size for RPB5/POLR2E is at 23-25 kDa.|Figure 2. IF analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody (A08765-1) and anti-Beta Tubulin antibody (M01857-3).
RPB5/POLR2E was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.|Figure 3. Flow Cytometry analysis of Hela cells using anti-RPB5/POLR2E antibody (A08765-1).
Overlay histogram showing Hela cells stained with A08765-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPB5/POLR2E Antibody (A08765-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPB5/POLR2E antigen affinity purified polyclonal antibody (A08765-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RPB5/POLR2E at approximately 23 kDa. The expected band size for RPB5/POLR2E is at 23-25 kDa.|Figure 2. IF analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody (A08765-1) and anti-Beta Tubulin antibody (M01857-3).
RPB5/POLR2E was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.|Figure 3. Flow Cytometry analysis of Hela cells using anti-RPB5/POLR2E antibody (A08765-1).
Overlay histogram showing Hela cells stained with A08765-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPB5/POLR2E Antibody (A08765-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-RPB5/POLR2E Antibody(A08765-1-50ul)
￥1180
