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Anti-CD36 Antibody (Clone#OTI3

F4)(MA01189-50ul)
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  • ¥1180
  • BOSTER已认证
  • MA01189-50ul
  • 中国
  • 2025年07月09日
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    13钻石会员
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    • 库存

      大量

    • 供应商

      广州威佳科技有限公司

    • 规格

      50ul

    产品概况

    货号 MA01189
    产品名称 Anti-CD36 Antibody (Clone#OTI3F4)
    基因名 CD36
    抗体来源 Mouse
    克隆 OTI3F4
    抗体亚型 Mouse IgG1
    分子量 88KD
    免疫原 Full length human recombinant protein of human CD36 (NP_000063) produced in HEK293T cell.
    内容 PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
    纯化方式 Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
    浓度 500 ug/ml
    产品形态 Liquid
    保存条件 Stable for 12 months from date of receipt. Store at -20°C as received.
    背景资料 The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
    研究类别 1. Tandon NN, Kralisz U, Jamieson GA (5 May 1989). "Identification of glycoprotein IV (CD36) as a primary receptor for platelet-collagen adhesion". J. Biol. Chem. 264 (13): 7576–83. PMID 2468670.2. Jump up ^ Silverstein RL, Baird M, Lo SK, Yesner LM (15 August 1992). "Sense and antisense cDNA transfection of CD36 (glycoprotein IV) in melanoma cells. Role of CD36 as a thrombospondin receptor". J. Biol. Chem. 267 (23): 16607–12. PMID 1379600.3. Kuda O, Pietka TA, Demianova Z, Kudova E, Cvacka J, Kopecky J, Abumrad NA (May 2013). "Sulfo-N-succinimidyl Oleate (SSO) Inhibits Fatty Acid Uptake and Signaling for Intracellular Calcium via Binding CD36 Lysine 164: SSO ALSO INHIBITS OXIDIZED LOW absorbance LIPOPROTEIN UPTAKE BY MACROPHAGES.". J. Biol. Chem. 288 (22): 15547–55. doi:10.1074/jbc.M113.473298.PMID 23603908.
    Uniprot ID CD36: P16671

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    [list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01189, Dilution 1:50)|Figure 2. Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-CD36mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA01189, Dilution 1:50)|Figure 3. HEK293T cells transfected with either overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-CD36 antibody, and then analyzed by flow cytometry (1:100).|Figure 4. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA01189) (1:500)|Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA01189) (1:500)|Figure 6. Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CD36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA01189) (1:500)|Figure 7. Anti-CD36 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD36 .|Figure 8. Western blot analysis of anti-CD36 antibody (MA01189). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human placenta tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD36 antigen affinity purified monoclonal antibody (MA01189) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.[/list_product_images]

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