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- BOSTER
- A30760-1-50ul
- 中国
- 2025年07月16日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A30760-1 |
|---|---|
| 产品名称 | Anti-SEPTIN8 Antibody |
| 基因名 | SEPTIN8 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 56KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of mouse Septin 8/Septin8 (RKAAMEALQSQALHATSQQPLRK). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Predicted to enable GTPase activity; SNARE binding activity; and molecular adaptor activity. Predicted to be involved in several processes, including regulation of SNARE complex assembly; regulation of intracellular protein transport; and regulation of protein stability. Located in myelin sheath. Part of septin complex. Is expressed in dermis; spinal cord ventricular layer; turbinate bone primordium; and ventral grey horn. Orthologous to human Septin8 (septin 8). |
| 研究类别 | 1. Blaser, S., Jersch, K., Hainmann, I., Wunderle, D., Zgaga-Griesz, A., Busse, A., Zieger, B. Human septin-septin interaction: CDCrel-1 partners with KIAA0202. FEBS Lett. 519: 169-172, 2002. 2. Blaser, S., Jersch, K., Hainmann, I., Zieger, W., Wunderle, D., Busse, A., Zieger, B. Isolation of new splice isoforms, characterization and expression analysis of the human septin SEPT8 (KIAA0202). Gene 312: 313-320, 2003.3. Nagase, T., Seki, N., Ishikawa, K., Ohira, M., Kawarabayasi, Y., Ohara, O., Tanaka, A., Kotani, H., Miyajima, N., Nomura, N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329, 1996. |
| Uniprot ID | SEPTIN8: Q8CHH9 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow Cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- Septin8 antibody (A30760-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: mouse brain tissue lysates.
Use rabbit anti- Septin8 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Septin8 at approximately 56KD. The expected band size for Septin8 is at 56KD.|Figure 2. Flow cytometry analysis of RAW264.7 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: rat C6 whole cell lysates,
Lane 2: mouse brain tissue lysates.
Use rabbit anti- Septin8 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Septin8 at approximately 56KD. The expected band size for Septin8 is at 56KD.|Figure 2. Flow cytometry analysis of RAW264.7 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SEPTIN8 Antibody(A30760-1-50ul)
￥1180
