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- BOSTER
- M02698-3-50ul
- 中国
- 2025年07月08日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M02698-3 |
|---|---|
| 产品名称 | Anti-SUB1 Antibody (monoclonal, 8D9D1) |
| 基因名 | SUB1 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 19KD |
| 免疫原 | E.coli-derived human PC4/SUB1 recombinant protein (Position: N62-L127). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs. |
| 研究类别 | 1. "Entrez Gene SUB1: SUB1 homolog (S. cerevisiae)".2. Kretzschmar M, Kaiser K, Lottspeich F, Meisterernst M (August 1994). "A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators". Cell 78 (3): 525–34.3. Ge H, Roeder RG (August 1994). "Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes". Cell 78 (3): 513–23. |
| Uniprot ID | SUB1: P53999 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SUB1 antibody (M02698-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: MCF-7 whole cell lysates,
Lane 4: T47D whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse spleen tissue lysates.
Use mouse anti- SUB1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SUB1 at approximately 19KD. The expected band size for SUB1 is at 14KD.|Figure 2. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of rat liver tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human laryngeal squamous cell carcinomatissue tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human testicular germ cell tumors tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human Lung adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 12. IF analysis using anti- SUB1 antibody (M02698-3).detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) and counterstained with DAPI (blue).|Figure 13. Flow cytometry analysis of HepG2 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: MCF-7 whole cell lysates,
Lane 4: T47D whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse spleen tissue lysates.
Use mouse anti- SUB1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SUB1 at approximately 19KD. The expected band size for SUB1 is at 14KD.|Figure 2. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of rat liver tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human laryngeal squamous cell carcinomatissue tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human testicular germ cell tumors tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human Lung adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SUB1 antibody (M02698-3). detected in paraffin-embedded section of human spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 12. IF analysis using anti- SUB1 antibody (M02698-3).detected in paraffin-embedded section of human intestinal cancer tissues. The tissue section were stained using the cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) and counterstained with DAPI (blue).|Figure 13. Flow cytometry analysis of HepG2 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SUB1 Antibody (monoclonal, 8D9D1)(M02698-3-50ul)
￥1180
